Abstract
Mesenchymal stem cells can effectively regenerate destroyed periodontal tissue. Because periodontal tissues are complex, mesenchymal stem cells that can differentiate into many tissue types would aid periodontal tissue regeneration. Indeed, periodontal tissue regeneration using mesenchymal stem cells derived from adipose tissue or bone marrow has been performed in experimental animal models, such as rat, canine, swine, and monkey. We have shown that rat periodontal tissue can be regenerated with adipose-derived stem cells. Adipose tissue contains a large number of stromal cells and is relatively easy to obtain in large quantities, and thus constitutes a very convenient stromal cell source. In this chapter, we introduce a rat periodontal tissue regeneration model using adipose-derived stem cells.
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References
Glickman, I., and Carranza, F. A. (1984) Glickman’s clinical periodontology. Philadelphia: Saunders 6th ed, 1–61.
Nyman, S., Lindhe, J., Karring, T., and Rylander, H. (1982) New attachment following surgical treatment of human periodontal disease. J Clin Periodontol 9, 290–6.
Lindhe, J., Pontoriero, R., Berglundh, T., and Araujo, M. (1995) The effect of flap management and bioresorbable occlusive devices in GTR treatment of degree III furcation defects. An experimental study in dogs. J Clin Periodontol 22, 276–83.
Hammarstrom, L. (1997) Enamel matrix, cementum development and regeneration. J Clin Periodontol 24, 658–68.
Sakallioglu, U., Acikgoz, G., Ayas, B., Kirtiloglu, T., and Sakallioglu, E. (2004) Healing of periodontal defects treated with enamel matrix proteins and root surface conditioning – an experimental study in dogs. Biomaterials 25, 1831–40.
Baelum, V., and Lopez, R. (2003) Weak evidence for a benefit of Emdogain in the treatment of intrabony defects. Evid Based Dent 4, 66.
Lopez, R., and Baelum, V. (2003) Is grafting biomaterials or biological agents more effective than open-flap debridement in treating deep intraosseous defects? Evid Based Dent 4, 64–5.
Kawaguchi, H., Hirachi, A., Hasegawa, N., Iwata, T., Hamaguchi, H., Shiba, H., et al. (2004) Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells. J Periodontol 75, 1281–7.
Yamada, Y., Ueda, M., Hibi, H., and Baba, S. (2006) A novel approach to periodontal tissue regeneration with mesenchymal stem cells and platelet-rich plasma using tissue engineering technology: a clinical case report. Int J Periodontics Restorative Dent 26, 363–9.
Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., et al. (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7, 211–28.
Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., et al. (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13, 4279–95.
Ogawa, R., Mizuno, H., Watanabe, A., Migita, M., Hyakusoku, H., and Shimada, T. (2004) Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice-including relationship of sex differences. Biochem Biophys Res Commun 319, 511–7.
Safford, K. M., Hicok, K. C., Safford, S. D., Halvorsen, Y. D., Wilkison, W. O., Gimble, J. M., et al. (2002) Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun 294, 371–9.
Tobita, M., Uysal, A. C., Ogawa, R., Hyakusoku, H., and Mizuno, H. (2008) Periodontal tissue regeneration with adipose-derived stem cells. Tissue Eng Part A 14, 945–53.
Seo, B. M., Miura, M., Gronthos, S., Bartold, P. M., Batouli, S., Brahim, J., et al. (2004) Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 364, 149–55.
Buser, D., Hoffmann, B., Bernard, J. P., Lussi, A., Mettler, D., and Schenk, R. K. (1998) Evaluation of filling materials in membrane-protected bone defects. A comparative histomorphometric study in the mandible of miniature pigs. Clin Oral Implants Res 9, 137–50.
Hammarstrom, L., Heijl, L., and Gestrelius, S. (1997) Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 24, 669–77.
Acknowledgments
The authors would like to thank Ms. Miyuki Takatori and Mr. Hidemasa Takafuji, the Center for Life Science, Nippon Medical School, for their expert assistance with the animal experiments and histological analyses. This work was supported in part by the Japanese Ministry of Education, Sports, and Culture (Grant number: 17591878).
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Tobita, M., Mizuno, H. (2011). Adipose-Derived Stem Cells for Periodontal Tissue Regeneration. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_34
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DOI: https://doi.org/10.1007/978-1-61737-960-4_34
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