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The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

In this chapter, we describe a method to isolate and to expand multipotent adipose-derived stem (hMADS) cells from human adipose tissue. We also describe culture conditions to differentiate them into adipocytes at a high rate. This culture system provides a powerful means for studying the first steps of human adipose cell development and a route for investigating effects of drugs on the biology of adipocytes. Finally, we provide a protocol to investigate gene function during proliferation and differentiation of hMADS cells by means of siRNA-mediated gene silencing approaches or forced expression by transducing hMADS cells permissive to infection with murine retrovirus vectors.

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Acknowledgments

This work was supported by Yves-Saint Laurent Beauté and CNRS funds.

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Authors and Affiliations

  1. Institut de Recherche, Signalisation, Biologie du Développement et Cancer, UMR6543 Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia-Antipolis, Nice, France

    Brigitte Wdziekonski, Tala Mohsen-Kanson, Phi Villageois & Christian Dani

Authors
  1. Brigitte Wdziekonski
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  2. Tala Mohsen-Kanson
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  3. Phi Villageois
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  4. Christian Dani
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Corresponding author

Correspondence to Christian Dani .

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Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

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Wdziekonski, B., Mohsen-Kanson, T., Villageois, P., Dani, C. (2011). The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_30

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_30

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

  • eBook Packages: Springer Protocols

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