Skip to main content
SpringerLink
Log in
Book cover

Adipose-Derived Stem Cells pp 29–36Cite as

Isolation of Murine Adipose-Derived Stem Cells

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Primary adipocytes grown in culture and derived from murine adipose tissue are essential for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stem cells along with a protocol for inducing adipogenesis in this cell population.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Kelly KA, Tanaka S, Baron R, Gimble JM. (1998) Murine bone marrow stromally derived BMS2 adipocytes support differentiation and function of osteoclast-like cells in vitro. Endocrinology 139:2092–2101.

    Article  PubMed  CAS  Google Scholar 

  2. Thompson DL, Lum KD, Nygaard SC, Kuestner RE, Kelly KA, Gimble JM, Moore EE. (1998) The derivation and characterization of stromal cell lines from the bone marrow of p53−/− mice: new insights into osteoblast and adipocyte differentiation. J Bone Miner Res 13:195–04.

    Article  PubMed  CAS  Google Scholar 

  3. Safford KM, Hicok KC, Safford SD, Halvorsen YD, Wilkison WO, Gimble JM, Rice HE. (2002) Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun 294:371–379.

    Article  PubMed  CAS  Google Scholar 

  4. Zheng B, Cao B, Li G, Huard J. (2006) Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chondrogenic differentiation in vivo. Tissue Eng 12:1891–1901.

    Article  PubMed  CAS  Google Scholar 

  5. Halvorsen YD, Bond A, Sen A, Franklin DM, Lea-Currie YR, Sujkowski D, Ellis PN, Wilkison WO, Gimble JM. (2001) Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis. Metabolism 50:407–413.

    Article  PubMed  CAS  Google Scholar 

  6. Bluher M, Patti ME, Gesta S, Kahn BB, Kahn CR. (2004) Intrinsic heterogeneity in adipose tissue of fat-specific insulin receptor knock-out mice is associated with differences in patterns of gene expression. J Biol Chem 279:31891–31901.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA

    Gang Yu, Xiying Wu & Jeffrey M. Gimble

  2. Ubiquitin Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA

    Gail Kilroy & Z. Elizabeth Floyd

  3. Center for Computational and Integrative Biology, Harvard Medical School, Boston, MA, USA

    Yuan-Di C. Halvorsen

Authors
  1. Gang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiying Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gail Kilroy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan-Di C. Halvorsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jeffrey M. Gimble
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Z. Elizabeth Floyd
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Yu, G., Wu, X., Kilroy, G., Halvorsen, YD.C., Gimble, J.M., Floyd, Z.E. (2011). Isolation of Murine Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-61737-960-4_3

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation