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Adipose-Derived Stem Cells pp 395–400Cite as

Soft Tissue Reconstruction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

The potential of adipose-derived stem cells (ASCs) in clinical applications of soft tissue regeneration is immense. This chapter discusses the isolation and characterization of human ASCs, expansion in vitro, and relevant in vivo models for adipose tissue engineering.

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References

  1. Finch, G. D. and Dawe, C. J. (2003) Hemiatrophy. J Pediatr Orthop 23, 99–101.

    PubMed  CAS  Google Scholar 

  2. Fokin, A. A. and Robicsek, F. (2002) Poland’s syndrome revisited. Ann Thorac Surg 74, 2218–25.

    Article  PubMed  Google Scholar 

  3. Neuber, F. (1893) Fettransplantation. Bericht uber die Verhandlungen der Deutscht Gesellsch Chir 22, 66.

    Google Scholar 

  4. Shenaq, S. M. and Yuksel, E. (2002) New research in breast reconstruction: adipose tissue engineering. Clin Plast Surg 29, 111–12.

    Article  PubMed  Google Scholar 

  5. Langstein, H. N. and Robb, G. L. (1999) Reconstructive approaches in soft tissue sarcoma. Semin Surg Oncol 17, 52–65.

    Article  PubMed  CAS  Google Scholar 

  6. Peer, L. A. (1956) The neglected free fat graft. Plast Reconstr Surg 18, 233–50.

    Article  CAS  Google Scholar 

  7. Crandall, D., Hausman, G., and Kral, J. (1997) A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microcirculation 4, 211–32.

    Article  PubMed  CAS  Google Scholar 

  8. Sierra-Honigmann, M. R., Nath, A. K., Murakami, C., García-Cardeña, G., Papapetropoulos, A., Sessa, W. C., Madge, L. A., Schechner, J. S., Schwabb, M. B., Polverini, P. J., and Flores-Riveros, J. R. (1998) Biological action of leptin as an angiogenic factor. Science 11, 1683–86.

    Article  Google Scholar 

  9. Patrick, C. W. J. (2001) Tissue engineering strategies for adipose tissue repair. Anat Rec 263, 361–66.

    Article  PubMed  CAS  Google Scholar 

  10. Nguyen, A., Pasyk, K. A., Bouvier, T. N., Hassett, C. A., and Argenta, L. C. (1990) Comparative study of survival of autologous adipose tissue taken and transplanted by different techniques. Plast Reconstr Surg 85, 378–86.

    Article  PubMed  CAS  Google Scholar 

  11. Boyce, R. G., Nuss, D. W., and Kluka, E. A. (1994) The use of autogenous fat, fascia, and nonvascularized muscle grafts in the head and neck. Otolaryngol Clin North Am 27, 39–68.

    PubMed  CAS  Google Scholar 

  12. Matsudo, P. K. and Toledo, L. S. (1988) Experience of injected fat grafting. Aesthetic Plast Surg 12, 35–38.

    Article  PubMed  CAS  Google Scholar 

  13. Rohrich, R. J., Sorokin, E. S., and Brown, S. A. (2004) In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg 113, 391–95.

    Article  PubMed  Google Scholar 

  14. Jackson, I. T., Simman, R., Tholen, R., and DiNick, V. D. (2001) A successful long-term method of fat grafting: recontouring of a large subcutaneous postradiation thigh defect with autologous fat transplantation. Aesthetic Plast Surg 25, 165–69.

    Article  PubMed  CAS  Google Scholar 

  15. Boschert, M., Beckert B. W., Puckett, C. L., and Concannon, M. J. (2002) Analysis of lipocyte viability after liposuction. Plast Reconstr Surg 109, 761–65.

    Article  PubMed  Google Scholar 

  16. Coleman, S. R. (2006) Structural fat grafting: more than a permanent filler. Plast Reconstr Surg 118, 108S–20S.

    Article  PubMed  CAS  Google Scholar 

  17. Peer, L. A. (1955) Cell survival theory versus replacement theory. Plast Reconstr Surg 16, 161–68.

    Article  CAS  Google Scholar 

  18. Gonzalez, A. M., Lobocki, C., Kelly, C. P., and Jackson, I. T. (2007) An alternative method for harvest and processing fat grafts: an in vitro study of cell viability and survival. Plast Reconstr Surg 120, 285–94.

    Article  PubMed  CAS  Google Scholar 

  19. Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., Alfonso, Z. C., Fraser, J. K., Benhaim, P., and Hedrick, M. H. (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13, 4279–95.

    Article  PubMed  CAS  Google Scholar 

  20. Gabbay, J. S., Heller, J. B., Mitchell, S. A., Zuk, P. A., Spoon, D. B., Wasson, K. L., Jarrahy, R., Benhaim, P., and Bradley, J. P. (2006) Osteogenic potentiation of human adipose-derived stem cells in a 3-dimensional matrix. Ann Plast Surg 57, 89–93.

    Article  PubMed  CAS  Google Scholar 

  21. Jin, X., Sun, Y., Zhang, K., Wang, J., Shi, T., Ju, X., and Lou, S. (2007) Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2. Biomaterials 28, 2994–3003.

    Article  PubMed  CAS  Google Scholar 

  22. Xu, Y., Balooch, G., Chiou, M., Bekerman, E., Ritchie, R. O., and Longaker, M. T. (2007) Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system. Biochem Biophys Res Commun 359, 311–6.

    Article  PubMed  CAS  Google Scholar 

  23. Rangappa, S., Entwistle, J. W., Wechsler, A. S., and Kresh, J. Y. (2003) Cardiomyocyte-mediated contact programs human mesenchymal stem cells to express cardiogenic phenotype. J Thorac Cardiovasc Surg 126, 124–32.

    Article  PubMed  CAS  Google Scholar 

  24. Rodríguez, L. V., Alfonso, Z., Zhang, R., Leung, J., Wu, B., and Ignarro, L. J. (2006) Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells. Proc Natl Acad Sci U S A 103, 12167–72.

    Article  PubMed  CAS  Google Scholar 

  25. Kokai, L. E., Rubin, J. P., and Marra, K. G. (2005) The potential of adipose-derived adult stem cells as a source of neuronal progenitor cells. Plast Reconstr Surg 116, 1453–60.

    Article  PubMed  CAS  Google Scholar 

  26. Safford, K. M., Hicok, K. C., Safford, S. D., et al. (2002) Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun 294, 371.

    Article  PubMed  CAS  Google Scholar 

  27. Casteilla, L., Planat-Bénard, V., Cousin, B., Silvestre, J. S., Laharrague, P., Charrière, G., Carrière, A., and Pénicaud, L. (2005) Plasticity of adipose tissue: a promising therapeutic avenue in the treatment of cardiovascular and blood diseases? Arch Mal Coeur Vaiss 98, 922–26.

    PubMed  CAS  Google Scholar 

  28. Planat-Bénard, V., Menard, C., André, M., Puceat, M., Perez, A., Garcia-Verdugo, J. M., Pénicaud, L., and Casteilla, L. (2004) Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res 94, 223–29.

    Article  PubMed  CAS  Google Scholar 

  29. Cao, Y., Sun, Z., Liao, L., Meng, Y., Han, Q., and Zhao, R. C. (2005) Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun 332, 370–9.

    Article  PubMed  CAS  Google Scholar 

  30. Rehman, J., Traktuev, D., Li, J., Merfeld-Clauss, S., Temm-Grove, C. J., Bovenkerk, J. E., Pell, C. L., Johnstone, B. H., Considine, R. V., and March, K. L. (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells Circulation 109, 1292–98.

    Article  PubMed  Google Scholar 

  31. Seo, M. J., Suh, S. Y., Bae, Y. C., and Jung, J. S. (2005) Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem Biophys Res Commun 328, 258–64.

    Article  PubMed  CAS  Google Scholar 

  32. Taléns-Visconti, R., Bonora, A., Jover, R., Mirabet, V., Carbonell, F., Castell, J. V., and Gómez-Lechón, M. J. (2006) Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells. World J Gastroenterol 12, 5834–45.

    PubMed  Google Scholar 

  33. Taléns-Visconti, R., Bonora, A., Jover, R., Mirabet, V., Carbonell, F., Castell, J. V., and Gómez-Lechón, M. (2007) Human mesenchymal stem cells from adipose tissue: differentiation into hepatic lineage. Toxicol In Vitro 21, 324–29.

    Article  PubMed  CAS  Google Scholar 

  34. Lu, F., Gao, J. H., Ogawa, R., Mizuro, H., and Hykusoku, H. (2006) Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice. Chin J Traumatol 9, 359–64.

    PubMed  Google Scholar 

  35. Choi, Y. S., Cha, S. M., Lee, Y. Y., Kwon, S. W., Park, C. J., and Kim, M. (2006) Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse. Biochem Biophys Res Commun 345, 631–7.

    Article  PubMed  CAS  Google Scholar 

  36. Halvorsen, Y. D., Bond, A., Sen, A., Franklin, D. M., Lea-Currie, Y. R., Sujkowski, D., Ellis, P. N., Wilkison, W. O., and Gimble, J. M. (2001) Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis. Metabolism 50, 407–413.

    Article  PubMed  CAS  Google Scholar 

  37. Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., and Hedrick, M. H. (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7, 211–22.

    Article  PubMed  CAS  Google Scholar 

  38. Lee, J. A., Parrett, B. M., Conejero, J. A., Laser, J., Chen, J., Kogon, A. J., Nanda, D., Grant, R. T., and Breitbart, A. S. (2003) Biological alchemy: engineering bone and fat from fat-derived stem cells. Ann Plast Surg 50, 610–17.

    Article  PubMed  Google Scholar 

  39. Wall, M. E., Bernacki, S. H., and Loboa, E. G. (2007) Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells. Tissue Eng 13, 1291–98.

    Article  PubMed  CAS  Google Scholar 

  40. Aksu, A. E., Rubin, J. P., Dudas, J. R., and Marra, K. G. (2008) Role of gender and anatomical region on induction of osteogenic differentiation of human adipose-derived stem cells. Ann Plast Surg 60, 306–22.

    Article  PubMed  CAS  Google Scholar 

  41. Schipper, B. M., Marra, K. G., Zhang, W., Donnenberg, A. D., and Rubin, J. P. (2008) Regional anatomic and age effects on cell function of human adipose-derived stem cells. Ann Plast Surg 60, 538–44.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thankfully acknowledge the Center for Biologic Imaging for CLSM/SEM and NIH R01CA114246-01A1 (JPR).

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Authors and Affiliations

  1. Division of Plastic Surgery, Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    J. Peter Rubin & Kacey G. Marra

Authors
  1. J. Peter Rubin
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  2. Kacey G. Marra
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Corresponding author

Correspondence to Kacey G. Marra .

Editor information

Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

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Rubin, J.P., Marra, K.G. (2011). Soft Tissue Reconstruction. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_28

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

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