Abstract
The versatile differentiation potential of adipose-derived stem cells (ASC) into cells of mesodermal, entodermal, and ectodermal origin places these cells at the forefront of cell-based therapies and cell transplantation. Epithelial differentiation of ASC may either be initiated by direct cell–cell or cell–matrix contacts, by chemical factors like retinoic acid, or via secreted cellular factors like cytokines, interleukins, or growth factors included in conditioned media.
This protocol describes methods to induce the in vitro differentiation of ASC from human adipose tissue into the epithelial lineage, and describes the methods used to verify this induced differentiation. We present two differentiation protocols based on either retinoic acid or conditioned medium of cultured epithelial cells.
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References
Gronthos S, Franklin DM, Leddy HA et al (2001) Surface protein charactzerization of human adipose-derived stromal cells. J Cell Physiol 189:54–63.
De Ugarte DA, Morizono K, Elbarbary A et al (2003) Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs 174:101–109.
Wagner W, Wein F, Seckinger A et al (2005) Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 33:1402–1416.
Kern S, Eichler H, Stoeve J et al (2006) Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord or adipose tissue. Stem Cells 24:1294–1301.
Zuk PA, Zhu M, Mizuno H et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228
Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295
Strem BM, Hicok KC, Zhu M et al (2005) Multilineage differentiation of adipose tissue-derived stem cells. Keio J Med 54:132–141.
Gimble JM, Guilak F (2003) Differential potential of adipose derived adult stem (ADAS) cells. Curr Top Dev Biol 58:137–160
Lombardo E, DelaRosa O, Mancheño-Corvo P et al (2009) Toll-like receptor-mediated signaling in human adipose-derived stem cells: implications for immunogenicity and immunosuppressive potential. Tissue Eng Part A 15:1579–1589
Stuart RO, Nigam SK (1995) Development of the tubular nephron. Semin Nephrol 15:315–326.
Brzoska M, Gauer S, Geiger H et al (2005) Epithelial differentiation of human adipose tissue-derived adult stem cells. Biochem Biophys Res Commun 330:142–150
Baer PC, Missler C, Bereiter-Hahn J et al (2009) Conditioned medium from renal tubular epithelial cells initiates differentiation of human mesenchymal stem cells. Cell Prolif 42:29–37
Mitchell JB, McIntosh K, Zvonic S, et al (2006) Immunophenotype of human adipose-derived cells: Temporal changes in stromal-associated and stem-cellassociated markers. Stem Cells 24:376–385
Baer PC, Nockher WA, Haase W et al (1997) Isolation of proximal and distal tubule cells from human kidney by immunomagnetic separation. Kidney Int 52:1321–1331
Baer PC, Bereiter-Hahn J, Schubert R et al (2006) Differentiation status of human renal proximal and distal tubular epithelial cells in vitro: Differential expression of characteristic markers. Cells Tissues Organs 184:16–22
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Baer, P.C., Brzoska, M., Geiger, H. (2011). Epithelial Differentiation of Human Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_21
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DOI: https://doi.org/10.1007/978-1-61737-960-4_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61737-959-8
Online ISBN: 978-1-61737-960-4
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