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Gel-Based and Gel-Free Proteomic Technologies

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Book cover Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 702))

Abstract

Proteomics refers to the analysis of expression, localization, functions, posttranslational modifications, and interactions of proteins expressed by a genome at a specific condition and at a specific time. Mass spectrometry (MS)-based proteomic methods have emerged as a key technology for unbiased systematic and high-throughput identification and quantification of complex protein mixtures. These methods have the potential to reveal unknown and novel changes in protein interactions and assemblies that regulate cellular and physiological processes. Both gel-based (one-dimensional [1D] gel electrophoresis, two-dimensional [2D] polyacrylamide gel electrophoresis, 2D difference in-gel electrophoresis [DIGE]) and gel-free (liquid chromatography [LC], capillary electrophoresis) approaches have been developed and utilized in a variety of combinations to separate proteins prior to mass spectrometric analysis. Detailed protocols for global proteomic analysis from adipose-derived stem cells (ASCs) using two central strategies, 2D-DIGE-MS and 2D-LC-MS, are presented here.

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Author information

Authors and Affiliations

  1. Proteomics and Metabolomics Core, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA

    Peter Scherp

  2. Protein Structural Biology, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA

    Ginger Ku, Liana Coleman & Indu Kheterpal

Authors
  1. Peter Scherp
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  2. Ginger Ku
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  3. Liana Coleman
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  4. Indu Kheterpal
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Corresponding author

Correspondence to Indu Kheterpal .

Editor information

Editors and Affiliations

  1. , Stem Cell Biology, Pennington Biomedical Research Center, Perkins Rd. 6400, Baton Rouge, 70808, Louisiana, USA

    Jeffrey M. Gimble

  2. School of Medicine, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112-2699, Louisiana, USA

    Bruce A. Bunnell

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Scherp, P., Ku, G., Coleman, L., Kheterpal, I. (2011). Gel-Based and Gel-Free Proteomic Technologies. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_13

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-959-8

  • Online ISBN: 978-1-61737-960-4

  • eBook Packages: Springer Protocols

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