Abstract
One of the hallmark characteristics of human adipose-derived stem cells (hASCs) is their ability to differentiate into cells of mesenchymal lineages. It is also becoming apparent that ASCs can mediate a therapeutic benefit through cytokine, paracrine-driven mechanisms influencing apoptosis, angiogenesis, and potent anti-inflammatory responses. Although there is still no clear consensus on the antigen expression pattern that will define hASCs, a protocol is also presented for the flow cytometric analysis utilizing a series of antibody panels. The analysis of these surface epitope patterns can aide in the isolation and characterization of hASCs. Moreover, using this standardized antibody panel, direct comparisons can be made between ASCs isolated from various tissue sources, which will benefit the field by providing uniformity to the comparison process.
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References
Rodbell, M. (1964) Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis, J. Biol. Chem. 239, 375–380.
Rodbell, M. (1966) Metabolism of isolated fat cells. II. The similar effects of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on glucose and amino acid metabolism, J. Biol. Chem. 241, 130–139.
Rodbell, M. (1966) The metabolism of isolated fat cells. IV. Regulation of release of protein by lipolytic hormones and insulin, J. Biol. Chem. 241, 3909–3917.
Rodbell, M. and Jones, A.B. (1966) Metabolism of isolated fat cells. The similar inhibitory action of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on lipolysis stimulated by lipolytic hormones and theophylline, J. Biol. Chem. 241, 140–142.
Zuk, P.A., Zhu, M, Ashjian, P., De Ugarte, D.A., Huang, J.I., Mizuno, H., Alfonso, Z.C., Fraser, J.K., Benhaim, P. and Hedrick, M.H. (2002) Human adipose tissue is a source of multipotent stem cells, Mol. Biol. Cell. 13, 4279–4295.
Engfeldt, P., Arner, P. and Ostman, J. (1980) Influence of adipocyte isolation by collagenase on phosphodiesterase activity and lipolysis in man, J. Lipid Res. 21, 443–448.
Deslex, S., Negrel, R., Vannier, C., Etienne, J. and Ailhaud, G. (1987) Differentiation of human adipocyte precursors in a chemically defined serum-free medium, Int. J. Obes. 11, 19–27.
Hauner, H., Entenmann, G., Wabitsch, M., Gaillard, D., Ailhaud, G., Negrel, R. and Pfeiffer, E.F. (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium, J. Clin. Invest. 84, 1663–1670.
Mitchell, J.B., McIntosh, K., Zvonic, S., Garrett, S., Floyd, Z.E., Kloster, A., Halvorsen, Y.D., Storms, R.W., Goh, B., Kilroy, G.S., Wu, X. and Gimble, J.M. (2006) The immunophenotype of human adipose derived cells: temporal changes in stromal- and stem cell-associated markers, Stem Cells 24, 376–385.
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Tucker, H.A., Bunnell, B.A. (2011). Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_10
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DOI: https://doi.org/10.1007/978-1-61737-960-4_10
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61737-960-4
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