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Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia

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Flow Cytometry Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 699))

Abstract

Flow cytometry immunophenotyping (FCI) of hematolymphoid specimens is a powerful tool in clinical medicine aiding in the accurate diagnosis and subclassification of acute and chronic leukemias, non-Hodgkin lymphomas, myelodysplasia, and other hematolymphoid neoplastic processes. Multiple flow cytometric strategies are used to evaluate hematolymphoid populations, including identification of neoplastic populations with aberrant immunophenotypes, abnormal maturation patterns, monotypic kappa/lambda light chain expression, restricted V-beta expression, and abnormal light scatter properties. In this chapter, we present a general approach to analyze hematolymphoid populations. Specimen handling, gating strategies, and appropriate antibody panels for flow cytometric detection of abnormal hematologic populations are presented.

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Author information

Authors and Affiliations

  1. Department of Laboratory Medicine, Hematology Section, National Institutes of Health Clinical Center, Bethesda, MD, USA

    Katherine R. Calvo

  2. Flow Cytometry Laboratory, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Catharine S. McCoy

  3. Flow Cytometry Unit, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Maryalice Stetler-Stevenson

Authors
  1. Katherine R. Calvo
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  2. Catharine S. McCoy
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  3. Maryalice Stetler-Stevenson
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Editor information

Editors and Affiliations

  1. Medical Center, Flow Cytometry Core Facility, George Washington University, I Street NW 2300, Washington, 20037, District of Columbia, USA

    Teresa S. Hawley

  2. Medical Center, Dept. Anatomy & Regenerative Biology, George Washington University, I Street NW 2300, Washington, 20037, District of Columbia, USA

    Robert G. Hawley

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Calvo, K.R., McCoy, C.S., Stetler-Stevenson, M. (2011). Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia. In: Hawley, T., Hawley, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology, vol 699. Humana Press. https://doi.org/10.1007/978-1-61737-950-5_14

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  • DOI: https://doi.org/10.1007/978-1-61737-950-5_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-949-9

  • Online ISBN: 978-1-61737-950-5

  • eBook Packages: Springer Protocols

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