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Mesenchymal Stem Cell Assays and Applications pp 443–457Cite as

Proteomic Analysis of Human Mesenchymal Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 698))

Abstract

Umbilical cord (UC) and placenta (P) are generally believed to be potential alternatives to bone marrow (BM), as sources of mesenchymal stem cells (MSC) for cell therapy. They possess immunophenotypic and functional characteristics which are similar to that of BM-MSC, yet one of the crucial factors in determining the tissue regeneration process – the migration capacity – is still unclear. In our previous study, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-fold higher than that of UC-MSC, respectively. By using 2-dimensional gel electrophoresis (2-DE) and combined MS and MS/MS analysis, six proteins were identified as differentially expressed among these MSC samples. Five out of the six proteins were known to be involved in cell migration as migration inhibiting or enhancing proteins.

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Acknowledgements

We would like to thank the donors of bone marrow, umbilical cord, and placenta. This work was supported by the Li Ka Shing Institute of Health Sciences Grant and RGC project: CUHK 7422_03M.

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Authors and Affiliations

  1. Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, P. R. China

    Hsiang-fu Kung

Authors
  1. Guo Li
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  2. Chu-yan Chan
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  3. Hua Wang
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  4. Hsiang-fu Kung
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Corresponding author

Correspondence to Hsiang-fu Kung .

Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Frederick, 21704, USA

    Mohan Vemuri

  2. Cellular Dynamics International, Inc., Science Drive 525, Madison, 53711, Wisconsin, USA

    Lucas G. Chase

  3. Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mahendra S. Rao

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© 2011 Springer Science+Business Media, LLC

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Li, G., Chan, Cy., Wang, H., Kung, Hf. (2011). Proteomic Analysis of Human Mesenchymal Stem Cells. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_32

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  • DOI: https://doi.org/10.1007/978-1-60761-999-4_32

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-998-7

  • Online ISBN: 978-1-60761-999-4

  • eBook Packages: Springer Protocols

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