Abstract
In this chapter, we describe the use of SuperHirn and MSight, two complementary tools developed to the processing of label-free LC/MS data in view of the quantitation of proteomics samples. While MSight is mainly dedicated to the visualisation and navigation into LC/MS data, SuperHirn is specialised in peak detection, normalisation and alignment of LC/MS runs. These two tools can be used in a complementary way and one of the possible usages is described here.
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References
Bantscheff M, Schirle M, Sweetman G, Rick J, Kuster B (2007) Quantitative mass spectrometry in proteomics: a critical review. Anal Bioanal Chem 389:1017–1031
America AH, Cordewener JH (2008) Comparative LC-MS: a landscape of peaks and valleys. Proteomics 8:731–749
Schulz-Trieglaff O, Pfeifer N, Gropl C, Kohlbacher O, Reinert K (2008) LC-MSsim – a simulation software for liquid chromatography mass spectrometry data. BMC Bioinform 9:423
Mueller LN, Brusniak MY, Mani DR, Aebersold R (2008) An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data. J Proteome Res 7:51–61
Palagi PM, Walther D, Quadroni M, Catherinet S, Burgess J, Zimmermann-Ivol CG, Sanchez JC, Binz PA, Hochstrasser DF, Appel RD (2005) MSight: an image analysis software for liquid chromatography-mass spectrometry. Proteomics 5:2381–2384
Appel RD, Palagi PM, Walther D, Vargas JR, Sanchez JC, Ravier F, Pasquali C, Hochstrasser DF (1997) Melanie II – a third-generation software package for analysis of two-dimensional electrophoresis images: I. Features and user interface. Electrophoresis 18:2724–2734
Mueller LN, Rinner O, Schmidt A, Letarte S, Bodenmiller B, Brusniak MY, Vitek O, Aebersold R, Muller M (2007) SuperHirn – a novel tool for high resolution LC-MS-based peptide/protein profiling. Proteomics 7:3470–3480
Rinner O, Mueller LN, Hubalek M, Muller M, Gstaiger M, Aebersold R (2007) An integrated mass spectrometric and computational framework for the analysis of protein interaction networks. Nat Biotechnol 25:345–352
Schmidt A, Gehlenborg N, Bodenmiller B, Mueller LN, Campbell D, Mueller M, Aebersold R, Domon B (2008) An integrated, directed mass spectrometric approach for in-depth characterization of complex peptide mixtures. Mol Cell Proteomics 7:2138–2150
Acknowledgment
MSight was partially funded by EU project LOCCANDIA (FP6-2004-IST-5 # 034202).
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Palagi, P.M., Müller, M., Walther, D., Lisacek, F. (2011). LC/MS Data Processing for Label-Free Quantitative Analysis. In: Hamacher, M., Eisenacher, M., Stephan, C. (eds) Data Mining in Proteomics. Methods in Molecular Biology, vol 696. Humana Press. https://doi.org/10.1007/978-1-60761-987-1_24
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DOI: https://doi.org/10.1007/978-1-60761-987-1_24
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