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Transgenic Mouse Methods and Protocols pp 57–73Cite as

Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 693))

Abstract

Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain a relatively high fertilization rate. This chapter attempts to show these procedures in simple terms.

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Author information

Authors and Affiliations

  1. Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan

    Naomi Nakagata

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  1. Naomi Nakagata
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Editors and Affiliations

  1. Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Antonius Deusinglaan 1, Groningen, 9713 AV, Netherlands

    Marten H. Hofker

  2. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Jan van Deursen

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Nakagata, N. (2011). Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_4

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  • DOI: https://doi.org/10.1007/978-1-60761-974-1_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-973-4

  • Online ISBN: 978-1-60761-974-1

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