Abstract
Introduction of germ line mutations in mice via genetic engineering involves alterations of the structure and characteristics of genes. These alterations are mostly introduced via molecular genetic technology either in embryonal stem cells or in one-cell stage embryos. This chapter describes classic biotechnological methods used to generate mice from modified pre-implantation embryos.
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Notes
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Tubes that have been incubated o/n at 55°C with SDS inside tend to leak when phenol–chloroform–isoamylalcohol is added.
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Voncken, J.W. (2011). Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_2
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DOI: https://doi.org/10.1007/978-1-60761-974-1_2
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