Abstract
Immune cells are derived from hematopoietic stem cells in the bone marrow. Experimental replacement of bone marrow offers the unique possibility to replace immune cells, to study gene function in mouse models of disease. Over the past decades, this technique has been used extensively to study, for instance, macrophage function in atherosclerosis. In this chapter, we describe the methods for both full bone marrow transplantations and T cell-specific transfers.
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http://www.protocol-online.net/index.htm (A collection of protocols for biomedical research including immunological research.)
http://tvmouse.compmed.ucdavis.edu/virtualNecropsy/ (A virtual mouse necropsy on the web including a schematic overview of the location of the various lymphnodes.)
http://www.rodentia.com/wmc/ (Everything about (mutant) mice.)
http://www.invitrogen.com/site/us/en/home/brands/Dynal.html (Vendor of immunomagnetic beads.)
http://www.jax.org/ (Vendor of (mutant) mouse strains.)
http://www.atcc.org/ (American Type Culture Collection.)
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de Winther, M.P.J., Heeringa, P. (2011). Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_19
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DOI: https://doi.org/10.1007/978-1-60761-974-1_19
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Publisher Name: Humana Press
Print ISBN: 978-1-60761-973-4
Online ISBN: 978-1-60761-974-1
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