Abstract
The mouse has become an important model for understanding human development, physiology and disease because of its genetic and biological similarity to humans. Desired mouse mutants with precise genetic alterations can now be generated through gene targeting in mouse embryonic stem cells. The rate-limiting factor in a gene-targeting experiment is the time needed for cloning to construct targeting vectors. The establishment of the Mutagenic Insertion and Chromosome Engineering Resource has made available targeting vectors for the insertional mutagenesis of a large number of mouse genes as well as for chromosome engineering throughout the mouse genome. This unique resource has enriched the repertoire of the genetic reagents for targeted manipulation of the mouse genome.
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References
Adams, D. J., Biggs, P. J., Cox, T., Davies, R., van der Weyden, L., Jonkers, J., Smith, J., Plumb, B., Taylor, R., Nishijima, I., Yu, Y., Rogers, J., and Bradley, A. (2004) Mutagenic insertion and chromosome engineering resource (MICER), Nat Genet 36, 867–871.
Zheng, B., Mills, A. A., and Bradley, A. (1999) A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice, Nucleic Acids Res 27, 2354–2360.
Auerbach, W., Dunmore, J. H., Fairchild-Huntress, V., Fang, Q., Auerbach, A. B., Huszar, D., and Joyner, A. L. (2000) Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines, Biotechniques 29, 1024–1028, 1030, 1032.
Bradley, A., Zheng, B., and Liu, P. (1998) Thirteen years of manipulating the mouse genome: a personal history, Int J Dev Biol 42, 943–950.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Analysis and cloning of eukaryotic genomic DNA, in Molecular cloning: a laboratory manual (Nolan, C., Ed.) 2nd ed., Cold Spring Harbor Laboratory Press, Plainview, New York.
Li, Z., Szurek, P. F., Jiang, C., Pao, A., Bundy, B., Le, W. D., Bradley, A., and Yu, Y. E. (2005) Neuronal differentiation of NTE-deficient embryonic stem cells, Biochem Biophys Res Commun 330, 1103–1109.
Mills, A. A., Zheng, B., Wang, X. J., Vogel, H., Roop, D. R., and Bradley, A. (1999) p63 is a p53 homologue required for limb and epiÂdermal morphogenesis, Nature 398, 708–713.
Zheng, B., Vogel, H., Donehower, L. A., and Bradley, A. (2002) Visual genotyping of a coat color tagged p53 mutant mouse line, Cancer Biol Ther 1, 433–435.
Cheah, S. S. and Behringer, R. R. (2000) Gene-targeting strategies, Methods Mol Biol 136, 455–463.
Hasty, P., Abuin, A., and Bradley, A. (1999) Gene targeting, principles, and practice in mammalian cells, in Gene targeting: a pracÂtical approach (Joyner, A. L., Ed.) 2nd ed., pp 1–35, Oxford University Press Inc., New York.
Ijuin, T., Yu, Y. E., Mizutani, K., Pao, A., Tateya, S., Tamori, Y., Bradley, A., and Takenawa, T. (2008) Increased insulin action in SKIP heterozygous knockout mice, Mol Cell Biol 28, 5184–5195.
Cleary, M. A., van Raamsdonk, C. D., Levorse, J., Zheng, B., Bradley, A., and Tilghman, S. M. (2001) Disruption of an imprinted gene cluster by a targeted chromosomal translocation in mice, Nat Genet 29, 78–82.
Mills, A. A. and Bradley, A. (2001) From mouse to man: generating megabase chroÂmosome rearrangements, Trends Genet 17, 331–339.
Ramirez-Solis, R., Liu, P., and Bradley, A. (1995) Chromosome engineering in mice, Nature 378, 720–724.
Yu, Y. and Bradley, A. (2001) Engineering chromosomal rearrangements in mice, Nat Rev Genet 2, 780–790.
Zheng, B., Mills, A. A., and Bradley, A. (2001) Introducing defined chromosomal rearrangements into the mouse genome, Methods 24, 81–94.
Simpson, E. M., Linder, C. C., Sargent, E. E., Davisson, M. T., Mobraaten, L. E., and Sharp, J. J. (1997) Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice, Nat Genet 16, 19–27.
Yu, Y. E., Morishima, M., Pao, A., Wang, D. Y., Wen, X. Y., Baldini, A., and Bradley, A. (2006) A deficiency in the region homologous to human 17q21.33-q23.2 causes heart defects in mice, Genetics 173, 297–307.
Li, Z., Yu, T., Morishima, M., Pao, A., LaDuca, J., Conroy, J., Nowak, N., Matsui, S., Shiraishi, I., and Yu, Y. (2007) Duplication of the entire 22.9-Mb human chromosome 21 syntenic region on mouse chromosome 16 causes cardiovascular and gastrointestinal abnormalities, Hum Mol Genet 16, 1359–1366.
Lange, U. C., Adams, D. J., Lee, C., Barton, S., Schneider, R., Bradley, A., and Surani, M. A. (2008) Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster, Mol Cell Biol 28, 4688–4696.
Acknowledgments
We thank Annie Pao and Paula Jones for their assistance. Work in the authors’ laboratory is supported by grants from the Louis Sklarow Memorial Fund, the Jerome Lejeune Foundation and the National Institutes of Health (R01HL91519).
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Liu, C., Szurek, P.F., Yu, Y.E. (2011). MICER Targeting Vectors for Manipulating the Mouse Genome. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_14
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DOI: https://doi.org/10.1007/978-1-60761-974-1_14
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