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Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines

  • François Lépine
  • Eric DézielEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 692)

Abstract

High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-l-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.

Key words

Bacteria Quorum sensing Acyl homoserine lactone 4-Hydroxy-2-alkylquinoline Analysis Quantification Mass spectrometry Liquid chromatography 

Notes

Acknowledgments

The important contribution of Sylvain Milot to the development of these methods is gratefully acknowledged. We also thank Émilie Gauthier for the help in the development of the AHLs quantification methods.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.INRS-Institut Armand-FrappierLavalCanada

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