Quorum Sensing pp 299-311 | Cite as

Generation of Quorum Quenching Antibodies

  • Gunnar F. KaufmannEmail author
  • Junguk Park
  • Alexander V. Mayorov
  • Diane M. Kubitz
  • Kim D. Janda
Part of the Methods in Molecular Biology book series (MIMB, volume 692)


The exchange of information within and among bacterial populations using small diffusible molecules has been termed “quorum sensing” (QS). Due to the extracellular distribution of the QS autoinducer molecules and the evolutionary highly conserved nature of signaling components, microbial QS systems represent an excellent target for anti-infective immunotherapy. Recently, we have described the generation of quorum quenching monoclonal antibodies (mAbs) against acyl homoserine lactones (AHL) used by Pseudomonas aeruginosa as well as Staphylococcal autoinducing peptides (AIP). These mAbs suppressed QS signaling in bacteria and neutralized AHL-mediated cytotoxic effects in vitro, as well as protected animals in Staphylococcus aureus infection models.

Key words

Quorum sensing Monoclonal antibodies Autoinducers Hybridoma Quorum quenching Pseudomonas aeruginosa Staphylococcus aureus 



This work was supported by the National Institutes of Health (AI080715 to G.F.K). This is manuscript number 20549 from The Scripps Research Institute.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Gunnar F. Kaufmann
    • 1
    Email author
  • Junguk Park
    • 1
  • Alexander V. Mayorov
    • 2
  • Diane M. Kubitz
    • 3
  • Kim D. Janda
    • 4
    • 1
  1. 1.Departments of Chemistry and Immunology & Microbial ScienceThe Scripps Research InstituteLa JollaUSA
  2. 2.The Skaggs Institute for Chemical Biology and Departments of Chemistry and Immunology & Microbial ScienceThe Scripps Research InstituteLa JollaUSA
  3. 3.Antibody Production Core FacilityThe Scripps Research InstituteLa JollaUSA
  4. 4.Worm Institute for Research and Medicine (WIRM)The Skaggs Institute for Chemical BiologyLa JollaUSA

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