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Quorum Sensing pp 173-187 | Cite as

Global Expression Analysis of Quorum-Sensing Controlled Genes

  • Martin SchusterEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 692)

Abstract

Transcript profiling has improved our understanding of quorum-sensing gene regulation on a global scale. Here, we describe methods for high-quality sample preparation for use in DNA microarray and real-time PCR experiments.

Key words

Transcriptome Microarray RNA isolation cDNA synthesis Real-time PCR Quorum sensing Pseudomonas aeruginosa 

Notes

Acknowledgements

Parts of the cDNA synthesis procedure are based on a protocol by Matt Wolfgang, University of North Carolina. Thanks to Joon-Hee Lee, University of Pusan, South Korea, for his suggestions on real-time PCR.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of MicrobiologyOregon State UniversityCorvallisUSA

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