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Quorum Sensing pp 133-145 | Cite as

Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules

  • Vladimir V. Kravchenko
  • Richard J. Ulevitch
  • Gunnar F. Kaufmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 692)

Abstract

Microbial pathogens use a wide repertoire of pathogen-associated molecular patterns (PAMPs) that affect host cell responses through activation of intracellular signaling events in a PAMP-specific manner. Here we describe a set of western blot-based methodologies for the evaluation of biochemical effects specifically induced by N-(3-oxo-acyl) homoserine lactones (3-oxo-AHLs) small molecules secreted by a number of Gram-negative bacteria, including the opportunistic human pathogen Pseudomonas aeruginosa. First, we will highlight the AHL-mediated effects on proapoptotic and stress pathways. Secondly, we will demonstrate that AHLs possess the ability to alter stimulus-induced NF-κB signaling, a key biochemical marker of inflammation and innate immune responses.

Key words

Acyl homoserine lactones Macrophages NF-kappa B Pseudomonas aeruginosa MAP kinase Apoptosis 

Notes

Acknowledgments

The authors would like to thank Colin A. Lowery for critical reading the manuscript. This work was supported by the National Institutes of Health (AI079436 to VVK and AI080715 to GFK). This is manuscript number 20550 from The Scripps Research Institute.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Vladimir V. Kravchenko
    • 1
  • Richard J. Ulevitch
    • 1
  • Gunnar F. Kaufmann
    • 2
  1. 1.Department of Immunology & Microbial ScienceThe Scripps Research InstituteLa JollaUSA
  2. 2.Departments of Chemistry and Immunology & Microbial ScienceThe Scripps Research InstituteLa JollaUSA

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