Abstract
A variety of stem cells, including embryonic, mesenchymal, and hematopoietic stem cells, have been isolated to date, resulting in the current investigation of many therapeutic applications. These stem cells offer a high potential in cell replacement therapies or in the regeneration of organ damage. One current obstacle in using these stem cells in clinical applications are the unknown or unexplained mechanisms regarding the activation of immune responses as well as their given potential of immune activity, which can attack the host tissue. Similarly, the unknown immunological environment, which can benefit tumor growth, also restrains the rapid clinical implementation of stem cells. We have shown that several techniques for measurement or illustration of immune responses in a hematopoietic murine CD4k/o mice transplantation model might be beneficial to get new insight into in vivo behavior of transplanted stem cells. Subjected to the transplantation setups (allogeneic, syngeneic, or xenogenic transplantation) different immune responses (enhancement of CD4+ T cells, cytokine activity) as well as different effects of the transplanted cells on the host organs (organ destruction, toxicity) are detectable. The methods used to describe such immune responses will be presented here.
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References
Zulewski, H. (2006) Stem cells with potential to generate insulin producing cells in man. Swiss. Med. Wkly. 136, 647–654.
Leker, R. R., and McKay, R. D. (2004) Using endogenous neural stem cells to enhance recovery from ischemic brain injury. Curr. Neurovasc. Res. 1, 421–427.
Ball, L. M., Bernardo, M. E., Roelofs, H., Lankester, A., Cometa, A., Egeler, R.M., et al. (2007) Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation. Blood 110, 2764–2767.
Wu, D., Boyd, A. S., and Wood, K. J. (2008) Embryonic stem cells and their differentiated derivatives have a fragile immune privilege, but still represent novel targets of immune attack. Stem Cells 26, 1939–1950.
Shlomchik, W. D. (2007) Graft-versus-host disease. Nat. Rev. Immunol. 7, 340–352.
Laub, R., Dorsch, M., Wenk, K., and Emmrich, F. (2001) Induction of immunologic tolerance to tetanus toxoid by anti-human CD4 in HLA-DR3(+)/human CD4(+)/murine CD4(−) multiple transgenic mice. Transplant. Proc. 33, 2182–2183.
Laub, R., Dorsch, M., Meyer, D., Ermann, J., Hedrich, H. J., and Emmrich, F. (2000) A multiple transgenic mouse model with a partially humanized activation pathway for helper T cell responses. J. Immunol. Methods. 246, 37–50.
Werksschrift, C. (1962). Ausgewählte Färbemethoden für Botanik, Parasitologie, Zoologie. Chroma-Ges. Schmid & Co., Stuttgart-Untertürkheim, p. 57.
De Simone, E. A., Saccodossi, N., Ferrari, A., and Leoni, J. (2008) Development of ELISAs for the measurement of IgM and IgG subclasses in sera from llamas (Lama glama) and assessment of the humoral immune response against different antigens. Vet. Immunol. Immunopathol. 126, 64–73.
Tung, T. H., Mohanakumar, T., and Mackinnon, S. E. (2005) TH1/TH2 cytokine profile of the immune response in limb component transplantation. Plast. Reconstr. Surg. 116, 557–566.
Ramanayake, T., Simon, D. A., Frelinger, J. G., Lord, E. M., and Robert, J. (2007) In vivo study of T-cell responses to skin alloantigens in Xenopus using a novel whole-mount immunohistology method. Transplantation 83, 159–166.
Jimenez, R., Ramirez, R., Carracedo, J., Aguera, M., Navarro, D., Santamaría, R. et al. (2005) Cytometric bead array (CBA) for the measurement of cytokines in urine and plasma of patients undergoing renal rejection. Cytokine 32, 45–50.
Carson, R. T., and Vignali, D. A. (1999) Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J. Immunol. Methods. 227, 41–52.
Beckmann, J. H., Yan, S., Lührs, H., Heid, B., Skubich, S., Förster, R., et al. (2004) Prolongation of allograft survival in ccr7-deficient mice. Transplantation 77, 1809–1814.
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Fricke, S. (2011). Measurement and Illustration of Immune Interaction After Stem Cell Transplantation. In: Nieden, N. (eds) Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases. Methods in Molecular Biology, vol 690. Humana Press. https://doi.org/10.1007/978-1-60761-962-8_21
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DOI: https://doi.org/10.1007/978-1-60761-962-8_21
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Online ISBN: 978-1-60761-962-8
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