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Analysis of Glycosaminoglycans in Stem Cell Glycomics

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Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 690))

Abstract

Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

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Acknowledgment

Our laboratory acknowledges generous support from the New York State Department of Health and the Empire State Stem Cell Board in the form of grant number N08G-264.

Author information

Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Boyangzi Li & Zhenqing Zhang

  2. Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Haiying Liu

  3. Departments of Chemistry and Chemical Biology and Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Hope E. Stansfield

  4. Departments of Biology and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Jonathan S. Dordick

  5. Department of Chemistry and Chemical Biology and Department of Biology and Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Robert J. Linhardt

Authors
  1. Boyangzi Li
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  2. Haiying Liu
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  3. Zhenqing Zhang
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  4. Hope E. Stansfield
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  5. Jonathan S. Dordick
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  6. Robert J. Linhardt
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Corresponding author

Correspondence to Robert J. Linhardt .

Editor information

Editors and Affiliations

  1. Dept. of Cell Biology & Neuroscience, University of California at Riverside, University Ave. 900, Riverside, 92521, California, USA

    Nicole I. Nieden

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Li, B., Liu, H., Zhang, Z., Stansfield, H.E., Dordick, J.S., Linhardt, R.J. (2011). Analysis of Glycosaminoglycans in Stem Cell Glycomics. In: Nieden, N. (eds) Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases. Methods in Molecular Biology, vol 690. Humana Press. https://doi.org/10.1007/978-1-60761-962-8_19

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  • DOI: https://doi.org/10.1007/978-1-60761-962-8_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-961-1

  • Online ISBN: 978-1-60761-962-8

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