Abstract
This protocol describes the construction of an automated imaging system and two assays for measuring avoidance behaviors in larval zebrafish. The first assay, called the “bouncing ball assay,” measures the response of larvae to a threatening stimulus displayed on an LCD screen. The second assay, called the “two-fish assay,” measures avoidance behavior of two siblings in a multiwell plate. The assays are robust and can easily be adapted for medium- to high-throughput applications.
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Acknowledgments
We thank Elena Carver, Emily Cole, Farrah Laliberte, and Charles Kambe for their help in testing the imaging system and image analysis protocols.
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Colwill, R.M., Creton, R. (2011). Automated Imaging of Avoidance Behavior in Larval Zebrafish. In: Kalueff, A., Cachat, J. (eds) Zebrafish Neurobehavioral Protocols. Neuromethods, vol 51. Humana Press. https://doi.org/10.1007/978-1-60761-953-6_3
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DOI: https://doi.org/10.1007/978-1-60761-953-6_3
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Online ISBN: 978-1-60761-953-6
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