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Intraperitoneal Injection as a Method of Psychotropic Drug Delivery in Adult Zebrafish

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Book cover Zebrafish Neurobehavioral Protocols

Part of the book series: Neuromethods ((NM,volume 51))

Abstract

Zebrafish behavioral phenotypes are often evaluated in response to pharmacological modulation by various psychotropic drugs. An important step in this process is the method of drug administration. While the most popular drug administration technique in zebrafish research is by immersion, systemic intraperitoneal injection is another effective alternative. This method is useful for drugs that are difficult to dissolve in water, or which require a better control over the amount of drug delivered to an individual animal. Here we outline a simple protocol for the intraperitoneal injection of drugs in adult zebrafish.

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Acknowledgments

The study was supported by Tulane university Intramural funds, the Gordon and the G. Lurcy Fellowships, Provost’s Scholarly Enrichment Fund, Newcomb Fellows Grant, LA Board of Regents Pfund, NARSAD YI award and Zebrafish Neuroscience Research Consortium (ZNRC).

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Authors and Affiliations

  1. Department of Pharmacology and Neuroscience Program, Zebrafish Neuroscience Research Consortium (ZNRC), Tulane University Medical School, New Orleans, LA, USA

    Adam Stewart

  2. Department of Pharmacology and Neuroscience Program, Tulane University Medical School, New Orleans, LA, USA

    Jonathan M. Cachat, Christopher Suciu, Peter C. Hart, Siddharth Gaikwad, Eli Utterback & John DiLeo

  3. Department of Pharmacology and Neuroscience Program, Tulane University Medical School, New Orleans, LA, 70112, USA

    Allan V. Kalueff

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  1. Adam Stewart
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  2. Jonathan M. Cachat
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  3. Christopher Suciu
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  4. Peter C. Hart
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  5. Siddharth Gaikwad
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  6. Eli Utterback
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  7. John DiLeo
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  8. Allan V. Kalueff
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Editors and Affiliations

  1. Neuroscience Program, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112, Louisiana, USA

    Allan V. Kalueff

  2. Neuroscience Program, Department of Pharmacology, Tulane University, Tulane Ave. 1430, New Orleans, 70112, Louisiana, USA

    Jonathan M. Cachat

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Stewart, A. et al. (2011). Intraperitoneal Injection as a Method of Psychotropic Drug Delivery in Adult Zebrafish. In: Kalueff, A., Cachat, J. (eds) Zebrafish Neurobehavioral Protocols. Neuromethods, vol 51. Humana Press. https://doi.org/10.1007/978-1-60761-953-6_14

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  • DOI: https://doi.org/10.1007/978-1-60761-953-6_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-952-9

  • Online ISBN: 978-1-60761-953-6

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