Abstract
Within the past 15 years, the utilisation of microarray technology for the detection of specific pathogen strains has increased rapidly. Presently, it is possible to simply purchase a pre-manufactured “off the shelf ” oligonucleotide microarray bearing a wide variety of known signature DNA sequences previously identified in the organism being studied. Consequently, a hybridisation analysis may be used to pinpoint which strain/s is present in any given clinical sample. However, there exists a problem if the study necessitates the identification of novel sequences which are not represented in commercially available microarray chips. Ideally, such investigations require an in situ oligonucleotide microarray platform with the capacity to synthesise microarrays bearing probe sequences designed solely by the researcher. This chapter will focus on the employment of the Combimatrix® B3 CustomArray™ for the synthesis of reusable, bespoke microarrays for the purpose of discerning multiple Human Papilloma Virus strains.
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Acknowledgments
The authors would like to thank Drs. Brooke P. Anderson, Mike Lodes, and Jeremy Dumsday from Combimatrix Corporation for their assistance in the realisation of this chapter.
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© 2011 Humana Press
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Ayers, D., Platt, M., Javad, F., Day, P.J.R. (2011). Human Papilloma Virus Strain Detection Utilising Custom-Designed Oligonucleotide Microarrays. In: Theophilus, B., Rapley, R. (eds) PCR Mutation Detection Protocols. Methods in Molecular Biology, vol 688. Humana Press. https://doi.org/10.1007/978-1-60761-947-5_7
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DOI: https://doi.org/10.1007/978-1-60761-947-5_7
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