Abstract
Venous thrombosis affects one in one thousand people each year, and in many countries, it is a major cause of morbidity and death in hospitalised patients. Factor V Leiden and the prothrombin c.20210G>A transition are relatively common in the Western World, and both increase the risk of venous thrombosis. The author describes the detection of t+++hese two genetic variants on the carousel-based Roche LightCycler®. This simple method has high sensitivity for DNA, making it possible to test blood samples without the need for traditional DNA extraction and purification.
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Cooper, P.C. (2011). Detection of Factor V Leiden and Prothrombin c.20210G>A Allele by Roche Diagnostics LightCycler® . In: Theophilus, B., Rapley, R. (eds) PCR Mutation Detection Protocols. Methods in Molecular Biology, vol 688. Humana Press. https://doi.org/10.1007/978-1-60761-947-5_16
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DOI: https://doi.org/10.1007/978-1-60761-947-5_16
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