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Targeting the Choroid Plexus-CSF-Brain Nexus Using Peptides Identified by Phage Display

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The Blood-Brain and Other Neural Barriers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 686))

Abstract

Drug delivery to the central nervous system requires the use of specific portals to enable drug entry into the brain and, as such, there is a growing need to identify processes that can enable drug transfer across both blood-brain and blood–cerebrospinal fluid barriers. Phage display is a powerful combinatorial technique that identifies specific peptides that can confer new activities to inactive particles. Identification of these peptides is directly dependent on the specific screening strategies used for their selection and retrieval. This chapter describes three selection strategies, which can be used to identify peptides that target the choroid plexus (CP) directly or for drug translocation across the CP and into cerebrospinal fluid.

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Acknowledgments

This work was supported in part by the National Institutes of Health (USA) and the Biochemistry and Biotechnology Research Council (UK). The authors would like to thank Drs. Paul Kassner and David Larocca who first engineered the initial EGF-displayed phage that helped establish the feasibility of CP targeting, Dr. Michael Burg who helped identify CP-targeting peptides in peptide libraries, and Ms. Emelie Amburn and Dr. Karen Sims who assisted in their characterization.

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Authors and Affiliations

  1. Department of Surgery, Division of Trauma, Burns and Critical Care, University of California San Diego, San Diego, CA, USA

    Andrew Baird & Brian P. Eliceiri

  2. Molecular Neuroscience Group, School of Medicine, University of Birmingham, Edgbaston, Birmingham, UK

    Ana Maria Gonzalez & Wendy Leadbeater

  3. Department of Clinical Neuroscience, Alpert Medical School at Brown University, Providence, RI, USA

    Conrad E. Johanson

  4. Department of Pathology, Alpert Medical School at Brown University, Providence, RI, USA

    Edward G. Stopa

Authors
  1. Andrew Baird
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  2. Brian P. Eliceiri
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  3. Ana Maria Gonzalez
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  4. Conrad E. Johanson
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  5. Wendy Leadbeater
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  6. Edward G. Stopa
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Editors and Affiliations

  1. Banting Institute, Dept. Laboratory Med. & Pathobiology, University of Toronto, College St. 100, Toronto, M5G 1L5, Ontario, Canada

    Sukriti Nag

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Baird, A., Eliceiri, B.P., Gonzalez, A.M., Johanson, C.E., Leadbeater, W., Stopa, E.G. (2011). Targeting the Choroid Plexus-CSF-Brain Nexus Using Peptides Identified by Phage Display. In: Nag, S. (eds) The Blood-Brain and Other Neural Barriers. Methods in Molecular Biology, vol 686. Humana Press. https://doi.org/10.1007/978-1-60761-938-3_25

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  • DOI: https://doi.org/10.1007/978-1-60761-938-3_25

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-937-6

  • Online ISBN: 978-1-60761-938-3

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