Abstract
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter inside the chloroplasts and to act as an electron acceptor for photosystem II.
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References
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Joly, D., Carpentier, R. (2011). Rapid Isolation of Intact Chloroplasts from Spinach Leaves. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_24
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DOI: https://doi.org/10.1007/978-1-60761-925-3_24
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Publisher Name: Humana Press, Totowa, NJ
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