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Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure–Function Characterization

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Photosynthesis Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 684))

Abstract

This chapter discusses the photosystem II (PSII) reconstitution into proteoliposomes. In the first part of the chapter, protocols are outlined for the preparation of lipid bilayer vesicles (liposomes) constituted of individual thylakoid lipids or their mixtures, for the preparation of PSII particles, and for the incorporation of the PSII particles into the liposomes. In the second part of the chapter, methodologies are described for the structure–function characterization of the PSII–lipid complexes (proteoliposomes). This includes the sodium dodecylsulfate-polyacrylamide gel electrophoresis determination of the PSII proteins, the measurement of oxygen-evolving activity of PSII in the proteoliposomes, the study of structural changes of the PSII proteins upon their incorporation into the lipid bilayers by Fourier transform infrared (FT-IR) spectroscopy, and the characterization of the PSII activity by fluorescence induction.

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Acknowledgments

This work was supported in different occasions by grants from the NSERC Canada, the Université du Québec à Trois-Rivières, the Département de chimie-biologie, and the Groupe de recherche en biologie végétale (GRBV).

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Authors and Affiliations

  1. Département de chimie biologie (GRBV), Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada

    David Joly, Sridharan Govindachary & Mário Fragata

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  1. David Joly
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  2. Sridharan Govindachary
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  3. Mário Fragata
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Correspondence to Mário Fragata .

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  1. , Département de Chimie Biologie, Université du Québec à Trois-Rivières, Boul. des Forges 3351, Trois-Rivieres, G9A5H7, Québec, Canada

    Robert Carpentier

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Joly, D., Govindachary, S., Fragata, M. (2011). Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure–Function Characterization. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_18

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  • DOI: https://doi.org/10.1007/978-1-60761-925-3_18

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