Abstract
We describe methods to isolate highly active oxygen-evolving photosystem II (PSII) membranes and core complexes from higher plants, and to purify subunits of the oxygen-evolving complex (OEC). The membrane samples used as the material for various in vitro studies of PSII are prepared by solubilizing thylakoid membranes with the nonionic detergent Triton X-100, and the core complexes are prepared by further solubilization of the PSII membranes with n-dodecyl-β-d-maltoside (β-DDM). The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline or salt treatment, and are then purified by ion-exchange chromatography using an automated high performance liquid chromatography system.
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References
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Yamamoto, Y., Leng, J., Shen, JR. (2011). Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins from Higher Plants. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_1
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DOI: https://doi.org/10.1007/978-1-60761-925-3_1
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Publisher Name: Humana Press, Totowa, NJ
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