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Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

  • Allan E. David
  • Arthur J. Yang
  • Nam Sun WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 679)

Abstract

While biocatalysts show tremendous potential for the industrial production of fine chemicals, their integration into large-scale processes has been slow. One of the main reasons for slow acceptance in industry is the inherent instability of the enzymes. Recent developments in bioengineering have shed some light on methods of improving enzyme stability. One method that has been used for many decades, successfully to varying degrees, has been the immobilization of enzymes. To this regards, silica gels have attracted much attention because of the ease of surface functionalization, high surface areas, mechanical and thermal stability, and resistance to both chemical and biological attack. We have previously shown the immobilization of invertase on silica gels with high immobilized activity and significantly improved stability. Here, we provide greater details on the methods for effecting the immobilization.

Key words

Enzyme immobilization Entrapment α-Amylase Silica gel Sol-gel Silicic acid Aminopropyltriethoxy-silane Glutaraldehyde Cross-linker 

Notes

Acknowledgments

This work was supported in part by NIH Grant R43 DK67723 and funds from Industrial Science and Technology Network (ISTN) Inc.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Allan E. David
    • 1
    • 2
  • Arthur J. Yang
    • 2
  • Nam Sun Wang
    • 1
    Email author
  1. 1.Department of Chemical and Biomolecular EngineeringUniversity of MarylandCollege ParkUSA
  2. 2.Industrial Science & Technology Network (ISTN) Inc.YorkUSA

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