Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems
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Recent pursuit on utilization of nanoscale materials has manifested a variety of configurations of highly efficient enzymic biocatalyst systems for biotechnological applications. Nanoscale structures are particularly powerful in effecting multienzyme biocatalysis. Inherent properties of nanomaterials – primarily, the high surface area to volume ratio and atomic scale 3D configurations – enable higher enzyme loadings, microenvironment control surrounding enzyme molecules, regulation on mass transfer, and protein structural stabilization of the biocatalyst as compared to traditional immobilization systems. This chapter introduces one versatile nanoscale immobilization method via details demonstrated using the case of nanoporous silica glass (30 nm diameter) for the concomitant incorporation of lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), and the cofactor (NADH).
Key wordsNanoporous carrier Mesoporous silica glass Covalent binding Coupling agent Cofactor regeneration NADH Lactate dehydrogenase Glucose dehydrogenase
- 1.Ansorge-Schumacher M.B. (2008) Immobilization of biological catalysts. Handbook of Heterogeneous Catalysis (2nd Edition) 1, 644–55. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.Google Scholar
- 13.El-Zahab B., Gonzalez D., Wang P. (2004) Dendrimer-supported multienzymatic biocatalysts with in situ cofactor regeneration. Abstracts of Papers, 228th ACS National Meeting, PMSE-037, Philadelphia, PA.Google Scholar
- 17.Roda A., Sabatini L., Barbieri A., Guardigli M., Locatelli M., Violante F.S., Rovati L.C., Persiani S. (2006) Development and validation of a sensitive HPLC-ESI-MS/MS method for the direct determination of glucosamine in human plasma. J Chromatogr , B: Analyt Technol Biomed Life Sci 844, 119–26.CrossRefGoogle Scholar