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Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

  • Nenad B. MilosavićEmail author
  • Radivoje M. Prodanović
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 679)

Abstract

One of the most used procedures for enzyme stabilization is immobilization. Although immobilization on solid supports has been pursued since the 1950s, there are no general rules for selecting the best support for a giving application. A macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate is a carrier consisting of macroporous beads for immobilizing enzymes of industrial potential for the production of fine chemicals and pharmaceuticals.

Key words

Poly (GMA-co-EGDMA) Macroporous Enzyme stabilization Covalent immobilization Glutaraldehyde Periodate 

Notes

Acknowledgments

We wish to thank Professor Slobodan M. Jovanović for the development and preparation of this new type of support for enzyme immobilization and stabilization as well as Professor Ratko M. Jankov for his helpful comments and information. This work was supported by the Ministry of Science of Serbia (projects no. 142020 and no. TP-20064).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Nenad B. Milosavić
    • 1
    Email author
  • Radivoje M. Prodanović
    • 2
  1. 1.Department of ChemistryBelgrade UniversityBelgradeSerbia
  2. 2.University of BelgradeBelgradeSerbia

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