Abstract
Regulatory macrophages (M regs) are a novel type of suppressor macrophage which may be a particularly suitable cell for inducing tolerance of solid organ transplants. In this article, we provide a detailed description of the generation of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols are provided for generating five comparator macrophage types which have been used as cell type-specificity controls in our work.
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References
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© 2010 Humana Press
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Hutchinson, J.A., Riquelme, P., Geissler, E.K., Fändrich, F. (2010). Human Regulatory Macrophages. In: Cuturi, M., Anegon, I. (eds) Suppression and Regulation of Immune Responses. Methods in Molecular Biology, vol 677. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-869-0_13
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DOI: https://doi.org/10.1007/978-1-60761-869-0_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-868-3
Online ISBN: 978-1-60761-869-0
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