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Myeloid-Derived Suppressor Cells: Characterization and Expansion in Models of Endotoxemia and Transplantation

  • Nicolas Van RompaeyEmail author
  • Alain Le Moine
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 677)

Abstract

CD11b+GR1+ myeloid-derived suppressor cells (MDSC) accumulate in several inflammatory conditions including cancer, infections, or trauma. MDSCs are found in bone marrow and lymphoid organs and suppress both innate and adaptive immune responses. Although mechanisms of suppression are not fully understood, they have been reported to require cell–cell contact and very often implicate l-arginine metabolism. We and others recently observed that lipopolysaccharide (LPS) administration, as other TLR ligands, induces MDSC. In this case, MDSC regulate immune response independently of l-arginine metabolism through heme oxygenase-1 activity. Manipulating MDSC as immunoregulators represents an attractive approach for cancer immunotherapy or transplantation. Herein, we describe methods for expanding and purifying MDSC, as well as in vitro and in vivo techniques to measure their suppressive functions.

Key words

Myeloid-derived suppressor cells Lipopolysaccharide T cells Transplantation Heme oxygenase-1 

Notes

Acknowledgment

Nicolas Van Rompaey is Research Fellow of the Fonds Erasme and Alain Le Moine is Research associate of the Fonds National de la Recherche Scientifique FNRS-Belgium.

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Copyright information

© Humana Press 2010

Authors and Affiliations

  1. 1.Institute for Medical ImmunologyUniversité Libre de BruxellesGosseliesBelgium

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