Preparation of Mouse Bone Marrow-Derived Dendritic Cells with Immunoregulatory Properties

  • Mercedes Segovia
  • Maria Cristina Cuturi
  • Marcelo HillEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 677)


Tolerogenic dendritic cells (Tol-DCs) are critical players in physiological tolerance. Moreover, they also play a role in immune regulation both in a pathophysiological context and when used as therapeutic tools in cell therapy strategies. Here, we describe a protocol to differentiate murine Tol-DCs from bone marrow precursors in vitro. Importantly, Tol-DCs actively suppress T cells stimulated with immunogenic allogeneic DCs. Indeed, Tol-DCs generated using this approach can be useful in studying and characterising the immunoregulatory pathways of Tol-DC and in developing Tol-DC-based cell therapy protocols using in vivo models.

Key words

Tolerogenic dendritic cells Mixed leukocyte reaction (MLR) Immunoregulation Bone marrow-derived DCs 



We are grateful to IMBIO, ESOT, Centaure, and RISET for financial support.


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Copyright information

© Humana Press 2010

Authors and Affiliations

  • Mercedes Segovia
    • 1
    • 2
    • 3
  • Maria Cristina Cuturi
    • 1
    • 2
    • 3
  • Marcelo Hill
    • 1
    • 2
    • 3
    Email author
  1. 1.INSERM UMR 643NantesFrance
  2. 2.Institut de Transplantation et de Recherche en Transplantation (ITERT)CHU NantesNantesFrance
  3. 3.Faculté de MédecineUniversité de NantesNantesFrance

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