Abstract
The embryonic zebrafish model offers the power of whole-animal investigations (e.g., intact organism, functional homeostatic feedback mechanisms, and intercellular signaling) with the convenience of cell culture (e.g., cost- and time-efficient, minimal infrastructure, small quantities of nanomaterial solutions required). The model system overcomes many of the current limitations in rapid to high-throughput screening of drugs/compounds and casts a broad net to evaluate integrated system effects rapidly. Additionally, it is an ideal platform to follow up with targeted studies aimed at the mechanisms of toxic action. Exposures are carried out in 96-well plates so minimal solution volumes are required for the assessments. Numerous morphological, developmental, and behavioral endpoints can be evaluated noninvasively due to the transparent nature of the embryos.
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Acknowledgments
The authors would like to thank the Sinhubber Aquatic Research Laboratory and the Environmental Health Sciences Center at Oregon State University where much of the protocols were developed. This work was supported by EPA STAR grant RD-833320 and NIEHS grants ES03850 and ES07060.
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Truong, L., Harper, S.L., Tanguay, R.L. (2011). Evaluation of Embryotoxicity Using the Zebrafish Model. In: Gautier, JC. (eds) Drug Safety Evaluation. Methods in Molecular Biology, vol 691. Humana Press. https://doi.org/10.1007/978-1-60761-849-2_16
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DOI: https://doi.org/10.1007/978-1-60761-849-2_16
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