Reflecting on Confocal Microscopy: A Personal Perspective

  • John White
Part of the Methods in Molecular Biology book series (MIMB, volume 1075)


The first practical laser scanning confocal microscopes were introduced to the biomedical community over 30 years ago. Their subsequent development continues to influence the introduction of new methods and applications of optical sectioning microscopy.

Key words

Confocal Laser scanning Optical section Fluorescence Living tissue Three-dimensional imaging 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • John White
    • 1
  1. 1.Laboratory for Optical and Computational Instrumentation and Laboratory of Molecular BiologyUniversity of WisconsinMadisonUSA

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