Abstract
Proteomic screening with small molecule microarrays can be a powerful tool in conjunction with various forward chemical genetics screening and high-throughput phenotype assays. Small molecule microarray screening can provide high quality information from the direct binding interaction between proteins of interest and a collection of small molecules in a high-throughput fashion. To realize this potential of small molecule microarray in the postgenomic era, the immobilization of small molecules on the surface of microscope glass slides has been a critical step, to apply small molecule library in protein screening assays and dissecting the protein network. In this chapter, we would like to focus on the protocol for the systematic immobilization of synthetic drug-like small molecules containing either specific functional handles or common functional groups.
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Acknowledgments
Our own investigations on proteomic screening and specific immobilization for small molecule microarray were supported by the National Research Foundation of Korea (NRF) and the WCU program through NRF funded by the Korean Ministry of Education, Science and Technology (MEST). These colleagues whose names are mentioned in the references have contributed to our understanding of the surface modification and high-throughput screening using small molecule microarray. Last but not least, we are grateful to all group members of chemical biology laboratory at Seoul National University for their generous support and assistance.
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Lee, H.Y., Park, S.B. (2010). Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules. In: Uttamchandani, M., Yao, S. (eds) Small Molecule Microarrays. Methods in Molecular Biology, vol 669. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-845-4_3
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DOI: https://doi.org/10.1007/978-1-60761-845-4_3
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