Abstract
One of the few available systems for gene expression in (hyper)thermophilic Archaea is the virus-based shuttle vector pMJ05 for Sulfolobus solfataricus. Although it is still not fully developed and there are some difficulties arising from the large size of the vector (>20 kb), it has successfully been used for the production of foreign and own proteins in S. solfataricus. Most often, the development of genetic tools for Archaea is held back by the lack of an efficient transformation system. In the case of pMJ05, this difficulty has been alleviated by using the Sulfolobus virus SSV1 as the vector backbone. The ability of the pMJ05 plasmid to spread in the culture as a virus, the availability of an effective selection marker (pyr) and of tunable promoters (araS and tf55α) make this system one of the first choices for heterologous gene expression in (hyper)thermophilic Archaea.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-60761-823-2_24
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-60761-823-2_24
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References
Allers, T. and Mevarech, M. (2005) Archaeal genetics – the third way. Nat Rev Genet 6, 58–73.
Kaczowka, S.J., Reuter, C.J., Talarico, L.A., and Maupin-Furlow, J.A. (2005) Recombinant production of Zymomonas mobilis pyruvate decarboxylase in the haloarchaeon Haloferax volcanii. Archaea 1, 327–334.
Heymann, J.A., Havelka, W.A., and Oesterhelt, D. (1993) Homologous overexpression of a light-driven anion pump in an archaebacterium. Mol Microbiol 7, 623–630.
Gardner, W.L. and Whitman, W.B. (1999) Expression vectors for Methanococcus maripaludis: overexpression of acetohydroxyacid synthase and beta-galactosidase. Genetics 152, 1439–1447.
Sato, T., Fukui, T., Atomi, H., and Imanaka, T. (2005) Improved and versatile transformation system allowing multiple genetic manipulations of the hyperthermophilic archaeon Thermococcus kodakaraensis. Appl Environ Microbiol 71, 3889–3899.
Sato, T., Fukui, T., Atomi, H., and Imanaka, T. (2003) Targeted gene disruption by homologous recombination in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol 185, 210–220.
Santangelo, T.J., Cubonova, L., and Reeve, J.N. (2008) Shuttle vector expression in Thermococcus kodakaraensis: contributions of cis elements to protein synthesis in a hyperthermophilic archaeon. Appl Environ Microbiol 74, 3099–3104.
Lucas, S., Toffin, L., Zivanovic, Y., Charlier, D., Moussard, H., Forterre, P., et al. (2002) Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi. Appl Environ Microbiol 68, 5528–5536.
Albers, S.V., Jonuscheit, M., Dinkelaker, S., Urich, T., Kletzin, A., Tampe, R., et al. (2006) Production of recombinant and tagged proteins in the hyperthermophilic archaeon Sulfolobus solfataricus. Appl Environ Microbiol 72, 102–111.
Jonuscheit, M., Martusewitsch, E., Stedman, K.M., and Schleper, C. (2003) A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector. Mol Microbiol 48, 1241–1252.
Aucelli, T., Contursi, P., Girfoglio, M., Rossi, M., and Cannio, R. (2006) A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus. Nucleic Acids Res 34, e114.
Palm, P., Schleper, C., Grampp, B., Yeats, S., McWilliam, P., Reiter, W.D., et al. (1991) Complete nucleotide sequence of the virus SSV1 of the archaebacterium Sulfolobus shibatae. Virology 185, 242–250.
Stedman, K.M., Schleper, C., Rumpf, E., and Zillig, W. (1999) Genetic requirements for the function of the archaeal virus SSV1 in Sulfolobus solfataricus: construction and testing of viral shuttle vectors. Genetics 152, 1397–1405.
Lubelska, J.M., Jonuscheit, M., Schleper, C., Albers, S.V., and Driessen, A.J. (2006) Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus. Extremophiles 10, 383–391.
Schleper, C., Kubo, K., and Zillig, W. (1992) The particle SSV1 from the extremely thermophilic archaeon Sulfolobus is a virus: demonstration of infectivity and of transfection with viral DNA. Proc Natl Acad Sci USA 89, 7645–7649.
Grant, S.G., Jessee, J., Bloom, F.R., and Hanahan, D. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci USA 87, 4645–4649.
Acknowledgements
The authors wish to thank Christa Schleper and Melanie Jonuscheit for the kind introduction to the pMJ05 system.
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Angelov, A., Liebl, W. (2010). Retracted Chapter: Heterologous Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus . In: Streit, W., Daniel, R. (eds) Metagenomics. Methods in Molecular Biology, vol 668. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-823-2_7
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DOI: https://doi.org/10.1007/978-1-60761-823-2_7
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