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Metagenomics pp 177–188Cite as

Screening for Cellulase-Encoding Clones in Metagenomic Libraries

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 668))

Abstract

Modern biotechnology has the steady need to continuously identify novel enzymes for use in biotechnological applications. In industrial applications, however, enzymes often have to function under extreme and nonnatural conditions (i.e., in the presence of solvents, high temperature and/or at extreme pH values). Cellulases have many industrial applications from the generation of bioethanol, a realistic long-term energy source, to the finishing of textiles. These industrial processes require cellulolytic activity under a range of pH, temperature, and ionic conditions, and they are usually carried out by mixtures of cellulases. Investigation of the broad diversity of cellulolytic enzymes involved in the natural degradation of cellulose is necessary for optimization of these processes.

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Authors and Affiliations

  1. Microbiology & Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Hamburg, Germany

    Nele Ilmberger & Wolfgang R. Streit

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  1. Nele Ilmberger
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  2. Wolfgang R. Streit
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Editors and Affiliations

  1. Biocenter Klein Flottbek, Microbiology & Biotechnology, University of Hamburg, Ohnhorststr. 18, Hamburg, 22609, Germany

    Wolfgang R. Streit

  2. Institute of Microbiology and Genetics, Dept. of Genomic & Applied Microbiology, Georg August University, Grisebachstraße 8, Göttingen, 37077, Germany

    Rolf Daniel

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Ilmberger, N., Streit, W.R. (2010). Screening for Cellulase-Encoding Clones in Metagenomic Libraries. In: Streit, W., Daniel, R. (eds) Metagenomics. Methods in Molecular Biology, vol 668. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-823-2_12

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  • DOI: https://doi.org/10.1007/978-1-60761-823-2_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-822-5

  • Online ISBN: 978-1-60761-823-2

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