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Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 666))

Abstract

Saliva harbors a wide spectrum of proteins that may reflect the health/disease status in the human body. Profiling of the proteins in saliva from a disease population can potentially yield valuable clinical parameters to be used for diagnosis and prognosis of the disease. Advances in proteomic technologies have enabled comprehensive profiling of protein expression in cells, tissue, and body fluids. When applied to readily accessible saliva samples from disease patients for biomarker study, such a global approach allows attaining the most discriminatory protein biomarkers that can best predict the disease status. In this chapter, we describe the protocols for proteomic analysis of saliva using 2D gel electrophoresis, Western blotting, and LC-MS/MS.

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Acknowledgments

This work was supported by the PHS grants U01-DE016275 (D.T.W.), R01-DE17593 (D.T.W.) R21-CA122806 (S.H.), and R03-DE017144 (S.H.).

Author information

Authors and Affiliations

  1. School of Dentistry, University of California Los Angeles, Los Angeles, CA, USA

    Shen Hu, Jiang Jiang & David T. Wong

  2. Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA, USA

    Shen Hu & David T. Wong

  3. Research and Diagnostic Systems, Inc, Los Angeles, CA, USA

    Jiang Jiang

  4. Division of Oral Biology and Oral Medicine, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  5. Dental Research Institute, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  6. Department of Craniofacial Biology, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  7. Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  8. Henry Samuel School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

Authors
  1. Shen Hu
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  2. Jiang Jiang
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  3. David T. Wong
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Editor information

Editors and Affiliations

  1. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Gregory J. Seymour

  2. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Mary P. Cullinan

  3. Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Nicholas C.K. Heng

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Hu, S., Jiang, J., Wong, D.T. (2010). Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 666. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-820-1_3

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  • DOI: https://doi.org/10.1007/978-1-60761-820-1_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-819-5

  • Online ISBN: 978-1-60761-820-1

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