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Oral Biology pp 21–30Cite as

Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 666))

Abstract

Saliva is an ideal translational research tool and diagnostic medium and is being used in novel ways to provide molecular biomarkers for a variety of oral and systemic diseases and conditions. The ability to analyze saliva to monitor health and disease is a highly desirable goal for oral health promotion and research. Saliva has been used to detect caries risk, periodontitis, oral cancer, breast cancer, salivary gland diseases, and systemic disorders such as hepatitis, HIV and HCV. Technology advancement has allowed high-throughput studies to be performed at a scale unrealized previously and is serving to advance the discovery and validation of salivary disease biomarkers. Of course, successful measurement of salivary analytes requires optimal collection, processing, and storage procedures and conditions. This chapter describes protocols for saliva collection, processing, and storage for the molecular analysis of salivary diagnostic constituents.

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Acknowledgment

This research is supported by NIH grants UO1-DE016275, UO1-DE017790, and RO1-DE017170.

Author information

Authors and Affiliations

  1. College of Dental Medicine, Western University of Health Sciences, Pomona, CA, USA

    Bradley Stephen Henson

  2. Division of Oral Biology and Oral Medicine, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  3. Dental Research Institute, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  4. School of Dentistry, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  5. Department of Craniofacial Biology, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  6. Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  7. Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

  8. Henry Samuel School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA, USA

    David T. Wong

Authors
  1. Bradley Stephen Henson
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  2. David T. Wong
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Editor information

Editors and Affiliations

  1. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Gregory J. Seymour

  2. Faculty of Dentistry, Department of Oral Sciences, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Mary P. Cullinan

  3. Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Great King Street 310, Dunedin, 9016, New Zealand

    Nicholas C.K. Heng

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Henson, B.S., Wong, D.T. (2010). Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 666. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-820-1_2

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  • DOI: https://doi.org/10.1007/978-1-60761-820-1_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-819-5

  • Online ISBN: 978-1-60761-820-1

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