Abstract
Selected techniques for the detection, quantification, and characterisation of HIV1, HIV2, and SIV, as applied to diagnostic and research purposes, are described. Representative nucleic acid testing protocols including nested PCR, RT-PCR, and quantitative real-time PCR, as well as protocols based on virus infectivity, are presented.
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Acknowledgements
We thank Claire Ham for expert laboratory skills and reading the manuscript and the members of our respective laboratories for sharing protocols.
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Berry, N., Herrera, C., Cranage, M. (2010). Detection, Quantification, and Characterisation of HIV/SIV. In: Stephenson, J., Warnes, A. (eds) Diagnostic Virology Protocols. Methods in Molecular Biology, vol 665. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-817-1_9
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DOI: https://doi.org/10.1007/978-1-60761-817-1_9
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