Resin Technologies: Construction and Staining of Resin TMA’s
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The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tissue microarrays created from it, provide good morphology but with a compromised antigenicity when compared to frozen tissue. In contrast, while solving the issue of antigenicity, frozen tissue suffers from a lack of morphology. We have demonstrated that tissue microarrays constructed in glycol methacrylate resin, when combined with a cold acetone fixation step, have been able to combine the superior morphology of resin-embedded sections with the superior antigenicity of frozen tissue for prospectively collected material.
Key wordsTissue microarray Glycol methacrylate Resin TMA Immunohistochemistry Acetone
The authors would like to acknowledge the technical staff of the immunohistochemistry group of the Atlas of Protein Expression and Histochemistry Research Unit for their contributions to the technical advancements.
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