Advertisement

Resin Technologies: Construction and Staining of Resin TMA’s

  • William J. HowatEmail author
  • Susan J. Wilson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 664)

Abstract

The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tissue microarrays created from it, provide good morphology but with a compromised antigenicity when compared to frozen tissue. In contrast, while solving the issue of antigenicity, frozen tissue suffers from a lack of morphology. We have demonstrated that tissue microarrays constructed in glycol methacrylate resin, when combined with a cold acetone fixation step, have been able to combine the superior morphology of resin-embedded sections with the superior antigenicity of frozen tissue for prospectively collected material.

Key words

Tissue microarray Glycol methacrylate Resin TMA Immunohistochemistry Acetone 

Notes

Acknowledgements

The authors would like to acknowledge the technical staff of the immunohistochemistry group of the Atlas of Protein Expression and Histochemistry Research Unit for their contributions to the technical advancements.

References

  1. 1.
    Beckstead JH, Halverson PS, Ries CA, Bainton DF. (1981) Enzyme histochemistry and immunohistochemistry on biopsy specimens of pathologic human bone marrow. Blood 57(6):1088–98.PubMedGoogle Scholar
  2. 2.
    Britten KM, Howarth PH, Roche WR. (1993) Immunohistochemistry on resin sections: a comparison of resin embedding techniques for small mucosal biopsies. Biotech Histochem 68(5):271–80.PubMedCrossRefGoogle Scholar
  3. 3.
    Glauert AM, Glauert RH, Rogers GE. (1956) A new embedding medium for electron microscopy. Nature 178(4537):803.PubMedCrossRefGoogle Scholar
  4. 4.
    Wilson SJ, Wallin A, Della-Cioppa G, Sandstrom T, Holgate ST. (2001) Effects of budesonide and formoterol on NF-kappaB, adhesion molecules, and cytokines in asthma. Am J Respir Crit Care Med 164(6):1047–52.PubMedGoogle Scholar
  5. 5.
    Casey TT, Cousar JB, Collins RD. (1988) A simplified plastic embedding and immunohistologic technique for immunophenotypic analysis of human hematopoietic and lymphoid tissues. Am J Pathol 131(2):183–9.PubMedGoogle Scholar
  6. 6.
    Gerrits P, Horobin R. (1996) Glycol methacrylate embedding for light microscopy: Basic principles and trouble-shooting. J Histotechnol 19(4):297–311.CrossRefGoogle Scholar
  7. 7.
    Howat WJ, Warford A, Mitchell JN, Clarke KF, Conquer JS, McCafferty J. (2005) Resin tissue microarrays: a universal format for immunohistochemistry. J Histochem Cytochem 53(10):1189–97.PubMedCrossRefGoogle Scholar
  8. 8.
    Yuan LC, Gulyas BJ. (1981) An improved method for processing single cells for electron microscopy utilizing agarose. Anat Rec 201(2):273–81.PubMedCrossRefGoogle Scholar
  9. 9.
    Hand N, Blythe D, Jackson P. (1996) Antigen unmasking using microwave heating on formalin fixed tissue embedded in methyl methacrylate. J Cell Pathol 1:31–7.Google Scholar
  10. 10.
    Takamiya H, Bodemer W, Vogt A. (1978) Masking of protein antigen by modification of amino groups with carbobenzoxychloride (benzyl chloroformate) and demasking by treatment with nonspecific protease. J Histochem Cytochem 26(11):914–20.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Histopathology/ISH facility, Li Ka Shing Centre, Cancer Research UKCambridge Research InstituteCambridgeUK
  2. 2.Histochemistry Research UnitSouthampton General HospitalSouthamptonUK

Personalised recommendations