Resin Technologies: Construction and Staining of Resin TMA’s

  • William J. HowatEmail author
  • Susan J. Wilson
Part of the Methods in Molecular Biology book series (MIMB, volume 664)


The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tissue microarrays created from it, provide good morphology but with a compromised antigenicity when compared to frozen tissue. In contrast, while solving the issue of antigenicity, frozen tissue suffers from a lack of morphology. We have demonstrated that tissue microarrays constructed in glycol methacrylate resin, when combined with a cold acetone fixation step, have been able to combine the superior morphology of resin-embedded sections with the superior antigenicity of frozen tissue for prospectively collected material.

Key words

Tissue microarray Glycol methacrylate Resin TMA Immunohistochemistry Acetone 



The authors would like to acknowledge the technical staff of the immunohistochemistry group of the Atlas of Protein Expression and Histochemistry Research Unit for their contributions to the technical advancements.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Histopathology/ISH facility, Li Ka Shing Centre, Cancer Research UKCambridge Research InstituteCambridgeUK
  2. 2.Histochemistry Research UnitSouthampton General HospitalSouthamptonUK

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