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From Gene to Clinic: TMA-Based Clinical Validation of Molecular Markers in Prostate Cancer

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Tissue Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 664))

Abstract

Current high-throughput screening techniques using DNA arrays have identified hundreds of new candidate biomarkers for diagnosis and risk prediction of prostate cancer. Large-scale analysis of clinical prostate cancer specimens is a key prerequisite for the validation of these genes. We have constructed a tissue microarray from more than 2,500 prostate cancers with full histo-pathological and clinical long-term follow-up data and analyzed expression and gene copy number patterns of 16 different candidate markers for their ability to predict prostate cancer progression and patient prognosis. The best candidates were used to extend established clinical prediction tools (nomograms) that were based on nonmolecular data only, such as prostate-specific antigene (PSA), clinical stage, and histological grading (Gleason grade). Using this approach, we could identify ANXA3 as an independent marker, which was capable of increasing the accuracy of the clinical nomogram, thereby fulfilling the criteria of a novel prognostic prostate cancer marker. This approach of integrating large-scale clinical and molecular variables may provide a new paradigm for the use of molecular profiling to predict the clinical outcome in prostate cancer.

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Author information

Authors and Affiliations

  1. Martini-Clinic, Prostate Cancer Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

    Thorsten Schlomm & Felix KH Chun

  2. Department of Pathology, Charité – University Medical Center, Berlin, Germany

    Andreas Erbersdobler

Authors
  1. Thorsten Schlomm
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  2. Felix KH Chun
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  3. Andreas Erbersdobler
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Corresponding author

Correspondence to Thorsten Schlomm .

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Editors and Affiliations

  1. Institute of Pathology, University Medical Ctr Hamburg-Eppendorf, Martinistrasse 52, Hamburg, 20246, Germany

    Ronald Simon

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Schlomm, T., Chun, F.K., Erbersdobler, A. (2010). From Gene to Clinic: TMA-Based Clinical Validation of Molecular Markers in Prostate Cancer. In: Simon, R. (eds) Tissue Microarrays. Methods in Molecular Biology, vol 664. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-806-5_17

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  • DOI: https://doi.org/10.1007/978-1-60761-806-5_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-805-8

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