DNA Copy Number Analysis on Tissue Microarrays

  • Anne KallioniemiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 664)


Detection of DNA sequence copy number changes is essential in both clinical practice and basic research, especially in cancer research. The combination of fluorescence in situ hybridization (FISH) and tissue microarray (TMA) technology provides high-throughput means for the evaluation of genetic aberrations in a large number of tissue samples. FISH on TMA is technically demanding and several protocols that include a variety of tissue pretreatment steps have been developed to improve the success of this methodology. Despite of the technical difficulties, FISH analysis on TMA has been successfully used not only to uncover genetic alterations in various malignancies but to also rapidly establish the clinical significance of such changes.

Key words

Tissue microarray High-throughput screening Copy number analysis Fluorescence in situ hybridization Molecular cytogenetics Molecular pathology Genetic aberrations 



The author would like to thank Ms. Eeva Laurila and Ms. Kati Rouhento for their assistance in preparing this article.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Institute of Medical TechnologyUniversity of Tampere and Tampere University HospitalTampereFinland

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