Skip to main content

Tissue Microarrays from Biopsy Specimens

  • Protocol
  • First Online:
Tissue Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 664))

Abstract

The construction of tissue microarrays from needle core biopsy specimens allows for the study of diseases with limited tissue samples and provides insight into tumors that are treated with nonsurgical approaches. While the techniques are technically challenging, in the hands of investigators they can reveal new insights into biomarkers of disease.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Zerbino, D.D. (1994) Biopsy: its history, current and future outlook. Lik Sprava. 3–4:1–9.

    PubMed  Google Scholar 

  2. Datta, M.W., Kahler, A., Macias, V., Brodzeller, T., Kajdacsy-Balla, A.A. (2005) A simple inexpensive method for the production of tissue microarrays from needle biopsy specimens: examples with prostate cancer. Appl Immunohistochem Mol Morphol. 13(1):96–103.

    Article  PubMed  Google Scholar 

  3. Jhavar, S., Corbishley, C.M., Dearnaley, D., Fisher, C., Falconer, A., Parker, C., Eeles, R., Cooper, C.S. (2005) Construction of tissue microarrays from prostate needle biopsy specimens. Br J Cancer. 93(4):478–82.

    Article  PubMed  CAS  Google Scholar 

  4. Obermann, E.C., Marienhagen, J., Stoehr, R., Wuensch, P.H., Hofstaedter, F. (2005) Tissue microarray construction from bone marrow biopsies. Biotechniques. 39(6):822, 824, 826.

    Article  PubMed  CAS  Google Scholar 

  5. Jhavar, S., Bartlett, J., Kovacs, G., Corbishley, C., Dearnaley, D., Eeles, R., Khoo, V., Huddart, R., Horwich, A., Thompson, A., Norman, A., Brewer, D., Cooper, C.S., Parker, C. (2009) Biopsy tissue microarray study of Ki-67 expression in untreated, localized prostate cancer managed by active surveillance. Prostate Cancer Prostatic Dis. 12(2):143–7.

    Article  PubMed  CAS  Google Scholar 

  6. Vergis, R., Corbishley, C.M., Norman, A.R., Bartlett, J., Jhavar, S., Borre, M., Heeboll, S., Horwich, A., Huddart, R., Khoo, V., Eeles, R., Cooper, C., Sydes, M., Dearnaley, D., Parker, C. (2008) Intrinsic markers of tumour hypoxia and angiogenesis in localised prostate cancer and outcome of radical treatment: a retrospective analysis of two randomised radiotherapy trials and one surgical cohort study. Lancet Oncol. 9(4):342–51. Epub 2008 Mar 17.

    Article  PubMed  Google Scholar 

  7. Singh, S.S., Mehedint, D.C., Ford, O.H. 3rd, Maygarden, S.J., Ruiz, B., Mohler, J.L. (2007) Feasibility of constructing tissue microarrays from diagnostic prostate biopsies. Prostate. 67(10):1011–8.

    Article  PubMed  Google Scholar 

  8. Rubin, M.A., Dunn, R., Strawderman, M., Pienta, K.J. (2002) Tissue microarray sampling strategy for prostate cancer biomarker analysis. Am J Surg Pathol. 26(3):312–9.

    Article  PubMed  Google Scholar 

  9. Kajdacsy-Balla, A.A., Geynisman, J.M., Macias, V., Setty, S., Nanaji, N.M., Berman, J.J., Dobbin, K., Melamed, J., Kong, X., Bosland, M., Orenstein, J., Bayerl, J., Becich, M.J., Dhir, R., Datta, M.W., Cooperative Prostate Cancer Tissue Resource. (2007) Practical aspects of planning, building, and interpreting tissue microarrays: the Cooperative Prostate Cancer Tissue Resource experience. J Mol Histol. 38(2):113–21.

    Article  PubMed  CAS  Google Scholar 

  10. DiVito, K.A., Charette, L.A., Rimm, D.L., Camp, R.L. (2004) Long term preservation of antigenicity in tissue microarrays. Lab Invest. 84:1071–8.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work has been supported in part by a grant from the National Cancer Institute (U01 CA 86473). We would like to thank the members of the Cooperative Prostate Cancer Tissue Resource, whose support and enthusiasm made the development of these techniques possible.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Milton W. Datta .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Datta, M.W., Kajdacsy-Balla, A.A. (2010). Tissue Microarrays from Biopsy Specimens. In: Simon, R. (eds) Tissue Microarrays. Methods in Molecular Biology, vol 664. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-806-5_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-806-5_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-805-8

  • Online ISBN: 978-1-60761-806-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics