Abstract
The construction of tissue microarrays from needle core biopsy specimens allows for the study of diseases with limited tissue samples and provides insight into tumors that are treated with nonsurgical approaches. While the techniques are technically challenging, in the hands of investigators they can reveal new insights into biomarkers of disease.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Zerbino, D.D. (1994) Biopsy: its history, current and future outlook. Lik Sprava. 3–4:1–9.
Datta, M.W., Kahler, A., Macias, V., Brodzeller, T., Kajdacsy-Balla, A.A. (2005) A simple inexpensive method for the production of tissue microarrays from needle biopsy specimens: examples with prostate cancer. Appl Immunohistochem Mol Morphol. 13(1):96–103.
Jhavar, S., Corbishley, C.M., Dearnaley, D., Fisher, C., Falconer, A., Parker, C., Eeles, R., Cooper, C.S. (2005) Construction of tissue microarrays from prostate needle biopsy specimens. Br J Cancer. 93(4):478–82.
Obermann, E.C., Marienhagen, J., Stoehr, R., Wuensch, P.H., Hofstaedter, F. (2005) Tissue microarray construction from bone marrow biopsies. Biotechniques. 39(6):822, 824, 826.
Jhavar, S., Bartlett, J., Kovacs, G., Corbishley, C., Dearnaley, D., Eeles, R., Khoo, V., Huddart, R., Horwich, A., Thompson, A., Norman, A., Brewer, D., Cooper, C.S., Parker, C. (2009) Biopsy tissue microarray study of Ki-67 expression in untreated, localized prostate cancer managed by active surveillance. Prostate Cancer Prostatic Dis. 12(2):143–7.
Vergis, R., Corbishley, C.M., Norman, A.R., Bartlett, J., Jhavar, S., Borre, M., Heeboll, S., Horwich, A., Huddart, R., Khoo, V., Eeles, R., Cooper, C., Sydes, M., Dearnaley, D., Parker, C. (2008) Intrinsic markers of tumour hypoxia and angiogenesis in localised prostate cancer and outcome of radical treatment: a retrospective analysis of two randomised radiotherapy trials and one surgical cohort study. Lancet Oncol. 9(4):342–51. Epub 2008 Mar 17.
Singh, S.S., Mehedint, D.C., Ford, O.H. 3rd, Maygarden, S.J., Ruiz, B., Mohler, J.L. (2007) Feasibility of constructing tissue microarrays from diagnostic prostate biopsies. Prostate. 67(10):1011–8.
Rubin, M.A., Dunn, R., Strawderman, M., Pienta, K.J. (2002) Tissue microarray sampling strategy for prostate cancer biomarker analysis. Am J Surg Pathol. 26(3):312–9.
Kajdacsy-Balla, A.A., Geynisman, J.M., Macias, V., Setty, S., Nanaji, N.M., Berman, J.J., Dobbin, K., Melamed, J., Kong, X., Bosland, M., Orenstein, J., Bayerl, J., Becich, M.J., Dhir, R., Datta, M.W., Cooperative Prostate Cancer Tissue Resource. (2007) Practical aspects of planning, building, and interpreting tissue microarrays: the Cooperative Prostate Cancer Tissue Resource experience. J Mol Histol. 38(2):113–21.
DiVito, K.A., Charette, L.A., Rimm, D.L., Camp, R.L. (2004) Long term preservation of antigenicity in tissue microarrays. Lab Invest. 84:1071–8.
Acknowledgments
This work has been supported in part by a grant from the National Cancer Institute (U01 CA 86473). We would like to thank the members of the Cooperative Prostate Cancer Tissue Resource, whose support and enthusiasm made the development of these techniques possible.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Datta, M.W., Kajdacsy-Balla, A.A. (2010). Tissue Microarrays from Biopsy Specimens. In: Simon, R. (eds) Tissue Microarrays. Methods in Molecular Biology, vol 664. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-806-5_11
Download citation
DOI: https://doi.org/10.1007/978-1-60761-806-5_11
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-805-8
Online ISBN: 978-1-60761-806-5
eBook Packages: Springer Protocols