Abstract
Molecular cytogenetics and especially fluorescence in situ hybridization (FISH) banding approaches are nowadays standard for the exact characterization of simple, complex, and cryptic chromosomal aberrations within the human genome. FISH-banding techniques are any kind of FISH techniques, which provide the possibility to characterize simultaneously several chromosomal subregions smaller than a chromosome arm. FISH banding methods fitting that definition may have quite different characteristics, but share the ability to produce a DNA-specific chromosomal banding. While the standard techniques such as G-bands by Trypsin using Giemsa banding lead to a protein-related black and white banding pattern, FISH-banding techniques are DNA-specific, more colorful, and thus, more informative. At present, the most frequently applied FISH banding technique is the multicolor banding (MCB/m-band) approach. MCB/m-band is based on region-specific microdissection libraries, producing changing fluorescence intensity ratios along the chromosomes. Here we describe the FISH-banding technique MCB/m-band and illustrate how to apply it for characterization of chromosomal breakpoints with a minimal number of FISH experiments.
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Acknowledgments
Supported in parts by the DFG (436 RUS 17/109/04, 436 RUS 17/22/06, WE 3617/2-1, 436 ARM 17/5/06, LI820/11-1, LI820/17-1), Boehringer Ingelheim Fonds, Evangelische Studienwerk e.V. Villigst, IZKF Jena (Start-up S16), IZKF together with the TMWFK (TP 3.7 and B307-04004), Stiftung Leukämie, and Stefan-Morsch-Stiftung.
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Liehr, T., Weise, A., Hinreiner, S., Mkrtchyan, H., Mrasek, K., Kosyakova, N. (2010). Characterization of Chromosomal Rearrangements Using Multicolor-Banding (MCB/m-band). In: Bridger, J., Volpi, E. (eds) Fluorescence in situ Hybridization (FISH). Methods in Molecular Biology, vol 659. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-789-1_16
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DOI: https://doi.org/10.1007/978-1-60761-789-1_16
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