Abstract
Gene modification of T cells with chimeric T-cell receptor (TCR) transgenes offers a novel way to generate tumor-specific T cells for cancer immunotherapy. Retroviruses have been utilized as the most common means of efficiently transducing primary T lymphocytes with these transgenes. In this section we describe methods for generation of chimeric TCR’s and utilization of retroviral vectors for efficient transduction of these transgenes in primary mouse T lymphocytes.
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Howland, L.J., Haynes, N.M., Darcy, P.K. (2010). Generation of Chimeric T-Cell Receptor Transgenes and Their Efficient Transfer in Primary Mouse T Lymphocytes. In: Yotnda, P. (eds) Immunotherapy of Cancer. Methods in Molecular Biology, vol 651. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-786-0_17
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DOI: https://doi.org/10.1007/978-1-60761-786-0_17
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