Abstract
Colloidal gold probes, including protein A-, IgG-F(ab’)2-, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A, IgG, and streptavidin.
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Yokota, S. (2010). Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab’)2, and Streptavidin. In: Schwartzbach, S., Osafune, T. (eds) Immunoelectron Microscopy. Methods in Molecular Biology, vol 657. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-783-9_8
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DOI: https://doi.org/10.1007/978-1-60761-783-9_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-782-2
Online ISBN: 978-1-60761-783-9
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