Abstract
Pre-embedding nanogold silver and gold intensification methods involve immunoreactions with nanogold-labeled antibodies and intensification of the nanogold particles before embedding and ultrathin sectioning. These highly sensitive methods show good resolution and ultrastructural preservation. They also are useful for simultaneous observation of immunolabeled cells under light and electron microscopes and for 3D immunoelectron microscopic analyses. Silver intensification is usually superior for immunolabeling. On the other hand, ultrastructural preservation is better when gold intensification is used. In this chapter, we introduce pre-embedding nanogold silver and gold intensification procedures for use primarily with cultured cells.
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Acknowledgments
The authors would like to thank Yuni Abe, Miho Ueda, Eri Toyama, and Yumiko Kosuga for technical assistance.
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Yamamoto, A., Masaki, R. (2010). Pre-embedding Nanogold Silver and Gold Intensification. In: Schwartzbach, S., Osafune, T. (eds) Immunoelectron Microscopy. Methods in Molecular Biology, vol 657. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-783-9_18
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DOI: https://doi.org/10.1007/978-1-60761-783-9_18
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Publisher Name: Humana Press, Totowa, NJ
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